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Hutson, Kristen H.

Nestorova, Gergana G.

Vazquez, Carolina

English

Louisiana Tech Digital Commons

Louisiana Tech UniversityLouisiana Tech Digital CommonsANS Research Symposium ANS Research Symposium 2019Apr 11th, 8:30 AM - 11:30 AMEffect of High-Energy Radiation on the Formationof 8- Hydroxy-DeoxyguanosineCarolina VazquezLouisiana Tech UniversityKristen H. HutsonLouisiana Tech UniversityGergana G. NestorovaLouisiana Tech UniversityFollow this and additional works at: https://digitalcommons.latech.edu/ans-research-symposiumThis Event is brought to you for free and open access by the Conferences and Symposia at Louisiana Tech Digital Commons. It has been accepted forinclusion in ANS Research Symposium by an authorized administrator of Louisiana Tech Digital Commons. For more information, please contactdigitalcommons@latech.edu.Recommended CitationVazquez, Carolina; Hutson, Kristen H.; and Nestorova, Gergana G., "Effect of High-Energy Radiation on the Formation of 8-Hydroxy-Deoxyguanosine" (2019). ANS Research Symposium. 40.https://digitalcommons.latech.edu/ans-research-symposium/2019/poster-presentations/40 35    Effect of high-energy radiation on the formation of 8- hydroxy-deoxyguanosine  Carolina Vazquez1, Kristen H. Hutson1, Gergana G. Nestorova 2  1Undergraduate student School of Biological Sciences, Louisiana Tech University 2Assistant Professor, School of Biological Sciences, Louisiana Tech University ___________________________________________________________________________ Reactive oxygen species (ROS) cause mutation of the DNA bases, which is implicated in carcinogenesis and variety of age-related disorders. 8-hydroxy 2′-deoxyguanine (8- OHdG) is a modified form of the guanine base that forms due to cellular exposure to ROS and is used as a biomarker for oxidative stress. Clinical radiation treatments expose cells to high levels of ROS, leading to an accelerated accumulation of 8-OHdG mutation. The objective of this study is to investigate the effect of high energy radiation on the rate of 8-OHdG formation in a human astrocytes. An enzyme-linked immunosorbent assay (ELISA) was performed in plate reader equipped with absorbance detection was used to quantify the level of 8-OHdG accumulation in cells treated with 0.5 Gy and 3 Gy proton and photon radiation to compare with normal, untreated cells. Reverse transcription quantitative PCR (RT-qPCR) analysis was applied to assess the mRNA expression levels of 8- oxoguanine glycosylase (OGG1) in both the treated and non-treated cells. OGG1 is an enzyme of the base excision repair pathway that main function is to remove 8-OHdG mutation. Decreased levels of OGG1 expression correlates with increased accumulation of 8-OHdG which leads to an increased level of cellular damage. In this study, human astrocytes were cultured and transported to Willis-Knighton Cancer Center in Shreveport for radiation treatment with 0.5 Gy and 3 Gy proton and photon radiation. The cells were incubated for 14 hours in humidity incubator and the analyzed. Cell media was collected and the concentration of oxidative damage by-product was analyzed using ELISA, with the results showing a steady increase of 8-OHdG accumulation as with the increased radiation dosage. The cells were lysed and RNA was purified using Qiagen total RNA purification kit. The results of this analysis reveal a strong trend of decreased levels of OGG1 expression as radiation dosage increased.         

Effect of High-Energy Radiation on the Formation of 8- Hydroxy-Deoxyguanosine

https://digitalcommons.latech.edu/cgi/viewcontent.cgi?article=1085&context=ans-research-symposium

Effect of High-Energy Radiation on the Formation of 8- Hydroxy-Deoxyguanosine

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