Rad predstavlja molekularnu analizu arheoloških ljudskih ostataka iz Zagajaca kraj Belišća u Hrvatskoj. U određivanje DNK uključeni su uzorak kosti iz pojedinačnog nalaza ljudske lubanje i dva zubna uzorka dobivena od zubā iz oskudnih ostataka pronađenih nešto dalje (svi su nalazi sekundarni). DNK smo analizirali po autosomnim kratkim udvojenim sljedovnim (STR) lokusima i amelogeninskom lokusu za spol. Cilj je bio utvrditi profile STR lokusa u smislu ispitivanja moguće molekularne sličnosti proučavanog materijala. Drugi cilj bio je molekularno utvrđivanje nepoznatog spola osobe predstavljene oskudnim ostacima, te potvrđivanje spola osobe koja je morfološki, na osnovi lubanje, određena kao žena. Iz zubnih smo uzoraka dobili oskudne/djelomične profile STR lokusa, dok je koštani uzorak iznjedrio potpuni izostanak STR lokusa. Samo se jedan alel (17) na lokusu D3S1358 pojavio u oba zubna uzorka. Međutim, molekularnim ispitivanjem po spolu potvrđena je nazočnost ženske osobe u oskudnim koštanim ostacima sukladno arheološkom nalazu. Znatni ili potpuni izostanak alela na STR lokusima upućuje na izraženo propadanje DNK. Potpun izostanak alela na svim lokusima u koštanom uzorku upućuje, također, na odsustvo onečišćenja suvremenom DNK.The paper presents molecular analysis of archaeological human remains from Zagajci near Belišće in Croatia. Bone sample from a single human cranium and two tooth samples obtained from teeth in a scanty “set” of remains found in farther vicinity (all secondary finds) were employed in DNA typing. DNA was analysed for autosomal short tandem repeat (STR) loci and the amelogenin – sex typing locus. The aim was obtaining STR loci profiles in order to investigate possible molecular relatedness between examined materials. Another aim was molecu¬lar identification of unknown sex of an individual represented by scanty remains, and confirming of the sex of the individual whose skull was estimated female by morphology. We obtained either low/partial STR loci allelic profiles for tooth samples, or a complete STR loci dropout for a bone sample. There was a single allele (17) of the D3S1358 locus shared among tooth samples. However, molecular sex identification confirmed the presence of a female individual in a scanty “set” of remains in accordance with archaeological data. Major or complete loci STR allele dropout is suggestive of a severe DNA degradation. A complete allelic loci dropout in a bone sample is also suggestive of the lack of contamination with modern DNA
