Post translational modifications (PTMs) are critical for regulating the function of

many proteins. PTMs are widespread in eukaryotic cells and catalysed by specific

enzymes. An important PTM is arginine methylation which is involved in many cellular

processes, such as RN

Fanis, P. (Pavlos)

Fanis, Pavlos

EUR Research Repository

English

Functional Proteomics Analysis of Transcription Factor Networks in Erythroid Cells

Post translational modifications (PTMs) are critical for regulating the function of

many proteins. PTMs are widespread in eukaryotic cells and catalysed by specific

enzymes. An important PTM is arginine methylation which is involved in many cellular

processes, such as RNA metabolism, DNA damage repair, DNA replication

and transcriptional regulation. Arginine methylation is catalysed by a family of enzymes

called protein arginine methyltransferases (PRMTs). PRMT1 is ubiquitously

expressed and is responsible for the majority of total protein arginine methylation

activity in mammalian cells. The focus of the research described in this thesis is

the isolation of Prmt1 associating proteins and the subsequent characterization of

Chromatin target of Prmt1 (Chtop), one of the identified interactors

Erasmus University Digital Repository

Stellingen behorende bij het proefschriftFunctional Proteomics Analysis of Transcription Factor Networks in Erythroid Cells1.      5FMC is a novel chromatin complex composed of Pelp1, Senp3, Las1L,            Wdr18 and Tex10. (this thesis)2.      The 5FMC complex interacts with methylated Chtop. (this thesis)3.      Prmt1 is the only type I arginine methyltransferase that methylates Chtop.                (this thesis)4.      Senp3 regulates the sumoylation status of Zbp-89. (this thesis)5.      LAS1L is localized in the nucleolus of human cells but can not be detected            in the nucleolus of mouse cells. (this thesis)6.      Sumoylation of transcriptional repressors is a general mechanism             to recruit chromatin remodeling and histone-modifying complexes. (Garcia-              Dominguez and Reyes, Biochimica et Biophysica Acta, 2009)7.      Histone sumoylation appears to govern chromatin structure, thus mediating            transcriptional repression. (Shiio and Eisenman, PNAs, 2003)8.      PELP1 is associated with SUMO-2 (Rosendorff et al., PNAs, 2006)9.      Methylation of arginine residues in proteins can provide a recognition          platform for interaction partners, or can modulate their biological activity            directly. (Bedford and Clarke, Mol. Cell, 2009)10.    Components of the 5FMC complex are involved in ribosome biogenesis               and RNA processing. (Finkbeiner et al., EMBO J., 2011 and Castle et al.,              Mol  Cell Biol., 2010)11.    “What is research but a blind date with knowledge”. (Will Harvey )                                                                                                                       Pavlos Fanis 

Functional proteomics analysis of transcription factor networks in erythroid cells

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