Alessandro P.S. de Moura

Amberg

Baddeley

Baxter

Bechhoefer

Berezney

Bressan

Conrad A. Nieduszynski

Conti

Czajkowsky

Dahmann

de Moura

Dekker

Dimitrova

Doksani

Duan

Falaschi

Friedman

Gibcus

Gillespie

Gustafsson

Hattersley

J. Julian Blow

Jackson

Jens Karschau

Kitamura

Knop

Knott

Leonhardt

Ligasová

Linskens

Lisby

Marek Gierliński

Marenduzzo

Marshall

Meister

Michaelis

Michelle Hawkins

Motulsky

Natsume

Nazan Saner

Nieduszynski

Pasero

Raghuraman

Renata Retkute

Retkute

Rippe

Rossi

Salic

Schermelleh

Sekedat

Sheff

Snaith

Sneppen

Straight

Struhl

Sutherland

Tanaka

Thomson

Tomoyuki U. Tanaka

Toyoaki Natsume

Tuduri

Yabuki

Yardimci

The Journal of Cell Biology

English

PubMed

Saner, Nazan

Karschau, Jens

Natsume, Toyoaki

Gierlinski, Marek

Retkute, Renata

Hawkins, Michelle

Nieduszynski, Conrad A

Blow, J Julian

de Moura, Alessandro P S

Tanaka, Tomoyuki U

The Open University

Stochastic association of neighboring replicons creates replication factories in budding yeast

Inside the nucleus, DNA replication is organized at discrete sites called replication factories, consisting of DNA polymerases and other replication proteins. Replication factories play important roles in coordinating replication and in responding to replication stress. However, it remains unknown how replicons are organized for processing at each replication factory. Here we address this question using budding yeast. We analyze how individual replicons dynamically organized a replication factory using live-cell imaging and investigate how replication factories were structured using super-resolution microscopy. Surprisingly, we show that the grouping of replicons within factories is highly variable from cell to cell. Once associated, however, replicons stay together relatively stably to maintain replication factories. We derive a coherent genome-wide mathematical model showing how neighboring replicons became associated stochastically to form replication factories, which was validated by independent microscopy-based analyses. This study not only reveals the fundamental principles promoting replication factory organization in budding yeast, but also provides insight into general mechanisms by which chromosomes organize sub-nuclear structures

Nieduszynski, Conrad A.

Blow, J. Julian

de Moura, Alessandro P. S.

Tanaka, Tomoyuki U.

Discovery Research Portal

Inside the nucleus, DNA replication is organized at discrete sites called replication factories, consisting of DNA polymerases and other replication proteins. Replication factories play important roles in coordinating replication and in responding to replication stress. However, it remains unknown how replicons are organized for processing at each replication factory. Here we address this question using budding yeast. We analyze how individual replicons dynamically organized a replication factory using live-cell imaging and investigate how replication factories were structured using super-resolution microscopy. Surprisingly, we show that the grouping of replicons within factories is highly variable from cell to cell. Once associated, however, replicons stay together relatively stably to maintain replication factories. We derive a coherent genome-wide mathematical model showing how neighboring replicons became associated stochastically to form replication factories, which was validated by independent microscopy-based analyses. This study not only reveals the fundamental principles promoting replication factory organization in budding yeast, but also provides insight into general mechanisms by which chromosomes organize sub-nuclear structures.</jats:p

Crossref

Gierliński, Marek

de Moura, Alessandro P.S.

University of East Anglia digital repository

https://aura.abdn.ac.uk/bitstream/2164/3009/1/J_Cell_Biol_2013_Saner_1001_12.pdf

Stochastic association of neighboring replicons creates replication factories in budding yeast

Abstract

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The Open University

Discovery Research Portal

Crossref

Discovery Research Portal

Crossref

University of East Anglia digital repository