To find out commonly used antibiotic combinations in the management of neonatal sepsis, prevalence of early onset sepsis (EOS) and late onset sepsis (LOS) in a public sector tertiary care hospital of Lahore. Neonatal sepsis is a clinical syndrome of bacteremia with systemic signs and symptoms of infection in the first 4 weeks of life. Neonatal sepsis is an important and common cause of morbidity and mortality in full term as well as preterm neonates. A retrospective observational study was conducted at the Neonatal Intensive Care Unit of a tertiary care hospital of Lahore from September 2013 to November 2013. 50 cases of neonatal sepsis suspected on clinical grounds were included in this study. LOS was more prevalent (60%) as compared to EOS (40%). Three combinations of antibiotics were frequently being used. Combination of Amikacin, Ampicillin & Ceftazidime was the most frequently used (48%) followed by combination of Amikacin, Ampicillin and Cefotaxime (30%), and combination of Vancomycin with Meropenam (22%). High potency antibiotics were being used frequently with less reliance on performance of culture tests for definitive therapy selection