Barack, Vered

Deblieck, Mathieu

Fathiukha, Andrii

Krugman, Tamar

Merchuck-Ovna, Lianne

Ordon, Frank

Perovic, Dragan

Pillen, Klaus

Saranga, Yehoshua

English

JKI Open Journal Systems (Julius Kühn-Institut)

10th Young Scientists Meeting, Siebeldingen, Germany, November 08-10, 2017 Deblieck et al. Anchoring the genetic map of near-isogenic introgression lines carrying wild emmer QTL-fragments for drought tolerance to the phys-ical map of Triticum diccocum Mathieu Deblieck1, Andrii Fathiukha2, Tamar Krugman2, Yehoshua Saranga3, Vered Barack3, LianneMerchuck-Ovnat3, Dragan Perovic1, Klaus Pillen4 and Frank Ordon1 1Julius Kühn-Institut, Institute for Resistance Research and Stress Tolerance, Quedlinburg 2Haifa University, Institute of Evolution, Haifa, Israel 3The Hebrew University of Jerusalem Faculty of Agriculture, Food and Environment, Rehovot, Israel 4Martin-Luther-University, Institute of Agricultural and Nutritional Sciences, Halle (Saale), Germany E-mail of corresponding author: mathieu.deblieck@jki.bund.deUntil now wheat breeders had to rely on genetic maps due to the lack of the ge-nome sequence. However, genetic and physical distances may differ significant-ly from each other, especially in centromeric and telomeric regions. This biased picture might lead to incorrect assumptions and estimations about the size and complexity of QTL-regions, the selection of suitable molecular markers and their suitability to transfer respec-tive QTLs into near-isogenic lines (NILs). The rising availability of cereal genomic sequences and their annotations deliv-ers a first standard that offers the possi-bility to dissolve this distorted picture and to get a better idea about the physi-cal size of QTL-regions and the number of genes involved. Suitable free open software packages that allow the comparison of genetic and physical data of NILs from their re-spective parents to our best knowledge doesn´t exist. We therefore developed a very simple Java-program that can be executed as a “.jar” file so that scientists without any programming-skills will be able to use it. Furthermore, the source code of the program will be published so that the code can easily be copied or implemented into other software pack-ages.  We tested the algorithm on two promis-ing NILs that carry QTL-regions for drought tolerance from wild emmer (T. diccocoides) on chromosome 2BS and 7AS. Both NILs suffer from linkage drag and were previously established with SSR markers that flank the regions of interest. Recently, these NILs, their re-spective recurrent parents and F6 descendents of the original mapping population were genotyped using the 15k-iSelect Illumina chip to improve the resolution of the previously calculated genetic map. This data and the availabil-ity of the physical wild emmer genome finally allowed us to get detailed infor-mation on these introgressions. The analysis revealed, e.g. that the centromeric regions of the wild emmer chromosomes were transmitted into both NILs. The physical segments of wild emmer cover significantly more than half of the corresponding physical size of the chromosomes. This may partly ex-plain the linkage drag in both NILs. We thank the German Ministry for Food and Agriculture for financial support. 50 Berichte aus dem Julius Kühn-Institut 192

Anchoring the genetic map of near-isogenic introgression lines carrying wild emmer QTLfragments for drought tolerance to the physical map of Triticum diccocum

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Anchoring the genetic map of near-isogenic introgression lines carrying wild emmer QTLfragments for drought tolerance to the physical map of Triticum diccocum

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