A simple protocol for in vitro shoot multiplication of 10 accessions including 8 varieties and 2advanced lines of short day Indian garlic (Allium sativum) is described. Two shootmultiplication media (Murashige and Skoog 1 and Murashige and Skoog 2) were tried usingbasal plate explant from mature cloves for multiple shoot induction. The medium (Murashigeand Skoog 2) consisting of 6-(γ,γ-dimethylallylamino)-purine (3 mg l-1) was the best forinduction of multiple shoots. Although genetic variability existed among the genotypes,shoot multiplication was obtained in all the accessions in Murashige and Skoog 2 mediumalong with almost 100% bulb formation in the shoots produced.
 
