The potential for photoautotrophic growth was studied in in vitro cultures of Chlorophytum borivilianum Sant. et Fernand. Best in vitro shoot differentiation was observed on MS medium supplemented with BAP 5 mg L-1, whereas MS + IBA 2 mg L-1 produced maximum root number and root length. The regenerated plantlets were then sequentially transferred to liquid basal medium having a gradual decrease in sucrose concentration [3%, 2%, 1.5%, 1%, 0.5% and 0%] after 48 hours stay in each. The plantlets thus formed were successfully hardened and transferred to sand-soil and farmyard manure mixture [1:1:1]. Approx. 90% of C. borivilianum regenerants survived after successful hardening
