Knowledge of fruit biochemical properties is very important for perception ofproduct behavior during harvesting, transportation, packaging, storing and regenerationprograms. In this study, physicochemical properties of fruit in 6 Iranian peach cultivars:‘Tak-daneh’, ’Zoodras’, ‘Anjiri-ye-khouni’, ‘Haj-kazemi’, ‘Zaferani’ and ‘Kosari’ weredetermined. For evaluation of antioxidant capacity and its relation with total phenolicsand flavonoids, total antioxidant capacity [measured with 3 methods: ferric-reducingantioxidant potential (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2-azinobis (3-ethylbenzothiazoline-6- sulfonic acid) radical scavenging assay (ABTS)] and totalphenolics and flavonoids in fruits extracts of aforementioned peach cultivars weremeasured and then, correlation of these parameters were evaluated. The highest °Brix anddry matter content among the studied cultivars corresponded to ‘Anjiri-ye-khouni’ andthe highest fruit humidity correlated to ’Zoodras’ and ‘Haj-kazemi’. The highest values ofvitamin C corresponded to ‘Anjiri-ye-khouni’ and ’Zoodras’ had the lowest vitamin C.Total ascorbic acid content was higher, then reported for other peach cultivars in the literature cited. Results revealed that ‘Anjiri-ye-khouni’ among the studied cultivars hadthe best antioxidant activity, flavonoid content and fruit quality attributes