Crotalaria pallida Aiton. Fabaceae has been used for the treatment of various diseases. The aim of the present study was to assess the enzymatic and non-enzymatic activities of Crotalaria pallida Aiton. The enzymatic antioxidant activity of in vivo leaves and in vitro callus was compared. The SOD shows high activity of (0.78±0.036) units/g tissue in in vivo leaves and Catalase shows high activity of (3.563±0.16) units/g tissue in in vitro callus. The non-enzymatic antioxidant properties of in vivo leaves and in vitro callus were evaluated using multiple assays. DPPH, Hydroxyl scavenging activity and Superoxide ion radical scavenging activity of in vivo leaves and in vitro callus were exhibited the IC50 values of 25±0.16 and 23.57±0.07, 90.18±3.57 and 77.41±2.5, 72.58±1.55 and 42.96±0.29 respectively. The total antioxidant activity showed increase with increase in concentration of both in vivo leaves and in vitro callus. The obtained results indicate that in vivo leaves and in vitro callus of Crotalaria pallida Aiton posses potent enzymatic activity and is a good source of non enzymatic antioxidants
