Anther culture is one of the most feasible techniques to obtain doubled haploid(DH) plants. In this study, anther culture responses of F2 derived bread wheat hybrids ofYektay406 × Altay 2000, Demir2000 × Soyer02, Atlı2002 × Müfitbey, Müfitbey × Atlı2002,Müfitbey × Eser, Müfitbey × Bezostaya were assessed to clarify the genotype-basedreactions under tissue culture conditions. Firstly, twenty five spikes which were grown indry land, collected from F2 hybrid plants. A total of 10.963 inoculated F2 anthers formed to188 callus in the primary hormone-free MN6 media. Some of the calli produced hybridgroups did not generate any haploid plantlet in regeneration media. Regenerationfrequencies of Demir2000 × Soyer02 and Müfitbey × Atlı2002 hybrids were 30.76% and63.63% respectively. F2 anthers of Müfitbey × Atlı2002 produced 121 callus, 10 albino and77 haploid plantlets. In contrary, Atlı2002 × Müfitbey produced only 26 callus, 3 albinoand zero haploid plantlets. Other corresponding hybrids of Müfitbey × Eser and Müfitbey× Bezostaya generated 9 and 13 callus, while the least number of callus was detected inYektay406 × Altay 2000. A correlation between callus number and haploid plantletnumber per 100 anther revealed a positive relationship for each F2 progeny (r = 0.988, P <0.01). Our results significantly indicated that Müfitbey cultivar might be used an effectiveanther source for DH wheat production
