The data obtained form routine urine analysis Viz physical examination, chemical examination and microscopic examination revealed that, in case of physical examination of urine sample is yellow to milky colour was observed while in case of appearance it was turbid to hazy where as putrefied odor was observed in all sample n=10. The data obtained form chemical examination indicates presence of albumin in all sample. Bile salt is present only one sample. The data obtained form microscopic examination revealed that pus cell count increases in all samples suffering from Urinary tract infection (uti)

and V.L. Deshmukh, A. M. Pande, R. T. Chavan, Muley S. S.

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Recent Research in Science and Technology 2011, 3(5): 38-39 ISSN: 2076-5061 www.recent-science.com  MEDICAL SCIENCES STUDIES ON ROUTINE URINE ANALYSIS OF URINARY TRACT INFECTION  A. M. Pande1∗, R. T. Chavan2, Muley S. S.3 and V.L. Deshmukh1    1Department of Microbiology, Toshniwal Art’s, Commerce & Science College, Sengaon, Dist. Hingoli – 431 513 (M.S.) 2Department of Botany, Toshniwal Art’s, Commerce & Science College, Sengaon, Dist. Hingoli – 431 513 (M.S.) 3Department of Dairy Science, Toshniwal Art’s, Commerce & Science College, Sengaon, Dist. Hingoli – 431 513 (M.S.)  Abstract The data obtained form routine urine analysis Viz physical examination, chemical examination and microscopic examination revealed that, in case of physical examination of urine sample is yellow to milky colour was observed while in case of appearance it was turbid to hazy where as putrefied odor was observed in all sample n=10. The data obtained form chemical examination indicates presence of albumin in all sample. Bile salt is present only one sample. The data obtained form microscopic examination revealed that pus cell count increases in all samples suffering from Urinary tract infection (uti). Keywords: Urine sample, E. coli, Candida albigus                                                          ∗ Corresponding Author, Email: aanandmpande@gmail.com Introduction The urinary tract is the body filtering system for removing liquid wastes or urine. Normally urinary tract is free forming any micro organism. But when the organism appears in urinary tract it is term as urinary tract infection. Urinary tract infection is an extremely common clinical problem. it is second commonest bacterial infection after respiratory diseases. Various factors contribute to uti like age, sex, season impairing defense mechanism to maintain sterility of urinary tract etc. Urinary tract infection is also associated with pylonephritis and cystitis. It may be symptomatic or obtain asymptomatic in pregnant women develops cystitis and 50 % develops pylonephritis Kass (1970). In children it leads to serious complications hence, it is very important to diagnose and treat infection to avoid such complications (Hadad, 2005).    Materials and Methods    Routine examination of urinary tract by chemical examination.  Determination of Protein 3 – 4 ml of centrifuge urine sample was transferred into a small test tube 10x75 mm. then 2 -3 drops of sulphosalisylic acid were added on the top of the sample, after 5 min the turbidity was observed for the presence of albumin (Protein).   Determination of Bile Salt 10 ml of urine sample was placed in a test tube then Dry sulphur powder was sprinkled on the top of the sample. Sink of sulphur powder at the bottom indicates presence of bile salt.   Determination of Glucose Mixture of 2 ml Benedict’s reagent and 8 drops of urine was heated for 5-10 min (Table-1) Table 1. Determination of sugar content of urine  Colour Conclusion Approx. Glucose mg/dl Blue Sugar absent Nil Green to Pale Yellow Sugar present in trace 200 – 500 Green to Dark Yellow Sugar present + to ++ 500 – 700 750 – 1000 Yellow ppt to orange Sugar present +++ 1000 – 1500 Orange to Red ppt Sugar present ++++ 1500 to more than 1500  Microscopic analysis of urine  Clean catch urine samples where collected in wind mouthed sterile container. Then the samples were transferred to centrifuge tube. The tubes were centrifuged at 2500rpm. For 5 minutes supernatant were poured off. One drop form deposit were placed on clean glass slide and covered with a cover slip. Then the slide was observed first under low power objective in subdued light. The contents of various were noted down E. Kumar et al./Rec Res Sci Tech 3 (2011) 38-39 Result and Discussion  The data obtained form routine urine analysis Viz physical examination, chemical examination and microscopic examination revealed that the pH reaction of all the samples shows acidic reaction. In case of physical examination of urine sample is yellow to milky colour was observed while in case of appearance it was turbid to hazy where as putrefied odor was observed in all sample n=10. The data obtained form chemical examination indicates presence of albumin in all sample. Bile salt is present in only one sample and sugar are absent in all samples suffering from UTI.In the reaction state albumin is present in urine samples this may be due to the reason that APN, renal failure, pyuria condition etc. The presence of albumin can be used as marker in progress in infections state. The data obtained from microscopic examination of revealed that pus cell count increases in all samples suffering from uti. The result of micro scopic of cast and crystal indicate that in only one sample epithelial cast and in one sample RBC cast was present. while, only one sample oxalate crystals was present. The result reveled that approximately all samples cell and yeast cell were present (Table 2). It might be due to urinary tract infection cause by pathogenic microorganism such as E. Coli, Candida etc.  Table 2 Routine Urine Analysis of urinary infection tract  References A. M., Al-Haddad, 2005. Urinary tract infection among pregnant women in Al-Mukalla district. yemen; Eastern Mediterranean Health Journal Vol.11 No. 3;  Kass E.H. (1970). Pregnancy, pyelonephritis and prematurity.Clinical Obstetrics and gynecology. Journal of Microbiology.13:239-54.    Sample Colour Reaction Odor Appearance Albumin Sugar Bile salt Pus cell WBC RBC Epithelial Cell Casts Crystal Bacteria/yeast 1 Pale yellow Acidic Putrified Hazzy Present Absent Absent 12-14 7-8 Absent 10-12 Absent Absent Occasional 2 Yellow Acidic Putrified Turbid Present Absent Absent 15-16 9-10 Absent 8-12 epi cast Absent Occasional 3 Pale yellow Acidic Putrified Turbid Present Absent Absent 8-10 6-8 Absent 10-12 Absent Absent Occasional 4 Red Acidic Putrified Hazzy Present Absent Absent 17-18 6-8 12-16 4-5 RBC Absent Occasional 5 Yellow Acidic Putrified Turbid Present Absent Absent 10-12 7-8 Absent 4-5 Absent Absent Occasional 6 Pale yellow Acidic Putrified Turbid Present Absent Absent 4-5 3-4 Absent 4-5 Absent Absent Occasional 7 Pale yellow Acidic Putrified Turbid Present Absent Absent 8-9 6-7 Absent 5-6 Absent Absent Occasional 8 Milky Acidic Putrified Cloudy Present Absent Absent 8-10 10-12 Absent 7-8 Absent Absent Occasional 9 Pale yellow Acidic Putrified Clear Present Absent Absent 3-4 2-3 Absent 2-3 Absent Absent Occasional 10 Pale yellow Acidic Putrified Turbid Present Absent Absent 7-8 3-4 Absent 3-4 Absent Absent Occasional 

Studies on Routine Urine Analysis of Urinary Tract Infection

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Studies on Routine Urine Analysis of Urinary Tract Infection

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