This study examined the effect of different pH, light intensity and different concentrations of NaNO3, NaH2PO4 and NaCl on the kinetics of growth, total carotenoids accumulated in Dunaliella salina EU5891200, a micro algal strain isolated from the salt pans of Tamil Nadu, South India. Results indicated that the highest amount of carotenoids detected grown at pH 8, 40 µEm-2s-1 light intensity had maximum production of total carotenoids. Lower salinity of 1.0 M NaCl favored high accumulation of total carotenoids. The amounts of total carotenoids recorded at 1.0 mM NaNO3 for D. salina had 20% more than the control. Phosphate was found to greatly influence the accumulation of total carotenoids (120.0µg/mL) in the isolate
