Ikeda  Minoru

Kanno  Minoru

Kijima  Akihiro

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Kijima, Akihiro

Ikeda, Minoru

Kanno, Minoru

Tohoku University Repository (TOUR) / 東北大学機関リポジトリ

Recent Research Topics in Genetic Approachesfor Sustainable Yield and Conservation ofMarine Organisms著者 Kijima  Akihiro, Ikeda  Minoru, Kanno  Minorujournal orpublication titleTohoku journal of agricultural researchvolume 59number 3-4page range 93-96year 2009-03URL http://hdl.handle.net/10097/44373TohokuJournalofAgriculturalResearchVoL59NQ.3-4,March2009pri●nteainJ⑫覗RecentResearchTopicsinGeneticApproachesforSustainableYieldandConservationofMarineOrganismsAkihiroKエJIMA,MinoruIKEDAandManamiKANNoLαborαtor3〆(ゾ∫ntegrαtive.AgUαttcBぢoJo931,FielaSciencaCenter,Go'(ZtluαteSche・e()fAgmbLegtuo噛alsei'en(re,T・h・kuUnivθe+s吻(Reoeived,March2,2009)SummaryInordertoachievesus七ainable丘sheriesintheworld,ithasbeenessen七ialtoinvestigatethegene右icsta右eofthebiologicalresourcesinnature,a、ndtOassessthegene七ictraitSforgenetiosandbreedinginarbificialprQduction.WefocusGn1)phy『10geneむicandpopulationgeneticstudiesonmollusca,crustacean,echinodermandfishwiththeconceptofthemanage皿en七〇f n ticresourees,and2)deteGもionofdelθteriousgenesexpressedthroughinbreedinginseleotivebreedingsアstemofPaGificabalone.PopulationgeneticstudiesonechinodermsforstockmαnαgementinJapαnEchinodermataisaminorfisherygroupintheworldexceptAsiancoun七ries,andthereislittlegeneticinformationforresouエcemanagementatpresent.Wehavebeentryingtomakethebasisofthepopulationgeneticstudyofthreeechinodeエrngroups,seacucumber,seaurchinandseastarfromthedevelopmentofDNAmarkers.Japaneseseacucum『ber,Apostichopausjupuoni(nLs,hasthreecolortypes,namelyRed,GreenandB}ack,howeverthegene七iGstatusofthemhaslongbeenuncertain.Weevaluatedthecolortraitquantitativelyandqualitatively,andrevealedthatRedwasclearlydividedfromtheothercolortypes,bothincolor-magni七udeandpigmenteomposition.Additionally,weinvestigatedthegeneticrelaもionshipamong七hevariantsbアaUozymeandmicrosatelliteDNAmarkers.Significan七geneもicdifferentiationwasnotobservedbe七weenGエeenandBlack,however,subs七antialgeneticdifferen七ia七ionwasdetectedbetweenRedandothertwovariantSeveninsym.patrア,TheseresulbSsuggesttha七theRedandother七wot)Tpesarereproductivelアisolated,whileGreenandBlaGkbelongtothesa皿egene9394A.砺 伽zαetα1.pooL.4ste?伽αpUectinzferaαn♂Asteoiasαmurensisarecommonseastarsdis七ributedalongthecoastofJapan.Theyhavesimilarlifehistorア 『butdif壬もrentph)dogeneticbackground・Comparativestudyontheirgeneticpopulationstruc-tureinferredfromtheallozアmeandmtDNAPCR-RFLPdatashowedquitecon七ractiveresul七,whichishomogeneousstrucもureofAsteWinαPUectinzfera,andhighlydifferentiaもedstructureof.4s古θ幅α8αmlLe'ensis.Thedifferenceinpopula-tionstruc七ureofthetwospecieswouldbecausedby七hedifferen.ceofcolonizationhistor)rsillcelastglaci乱lperiod.Theseresultswouldcontributetounderstandthehistorica}formationprocessofmarinecoastalcommuniもies.StudyongeneticpopulationstructureofspinykingcrabPαrα胱hodesbrevipesThespinykingcrabP伽・α薦1翻 θsbrevipes(Japanesename:Hanasaki-gani)occursoffnorthernJapanintheSeaofOkhoもskandintheBeringSea.ItisanimportantfisheryresourceoffeasternHokkaido,butthecatcheshavedeclinedinrecenもyears.NemuroCitアinHokkaido,wellknownfor丘sheryofthiscrab,hasorganizedthe"HanasakiProgram"consistingofresearcheエsfromdifferentstudyfields(ecolo9)τ,oceanography,andpopulationgerletics,e七c,)tosurveアthiscrab'sbiologyforもheresource皿anagementandstockenhanGementtowardsus七ainableuse.Wehavejoined七hispエogramsince2005inordertorevealpopulationstructureofthiscrabaroundcoas七alareasofHokkaidoandRussia.UsingnovelmicrosatelliteandmitochondrialDNAmarkers,werevealedthaもsignificantgeneticdifferentiationoccuエsbetweenthesou.thoftheSeaofOkhotsk(thesouthernSakhalinandnorthernHokka、ido)and七heeastofthePacificOcean(theSouthKuエilalldeasternHokkaido).TheboundarybetweenthesetwoputativepopulationsistheNemuエoStraight.Theseresultswθreexact1アcoincidentwiththepredictionofearliersimulationexperimentsontheplanktoniclarvaldriftbasedonthePOM(1)エincetonOceanModel)by・anotherresearchteamofthepエogram.ThisissuesアlnbolicallアindicatesthatGoupledmolecularpopulationgeneticandoceanphysicalmodelanal)Tsesgiveusessentialinformation,e.g.cryp七icbarエiertodispersalinocean,tOrecognizemanagemen七units(MUs)ofthemarineorganismswithlongPlanktonicperiod.InbreedingdepressionanddeleteriousgenesdetectedinPacificabalone`lnbreedingdepression'maybeanavoidablephenomenonforabaloneculture.However,oniyafewstudiesh、avebeencarriedoutoninbreedingdepression.InourlaboratOry,usingsixfamiliesproducedinl994,afaGtorialmatingsystemincludinginbreedingandoutbreedingwasconstructedinoエdertodemonstrateSustainαbility(ゾMα7ineO79α%盛8賜s95inbreedingdepressiontraitsofthePacificabalone,11α あo拓8discteshan?zαi.Significantdifferencesinferti五izationratθandgrowthwerenotobservedbetweeninbreedingandoutbreedingGエosses.However,thedeformiもyra七eofveligerlaエvaewasalwayshigherininbreedingcrosses七hanthatofoutbreedingcrossesinallexperi]〔nents,Moreover,asignificantlyhighdefor】[nityratewasobservedinsomefull-sibfa皿iliesofinbreeding,Alternatively,thesurvivalratesofinbreed-ingcrossesweremuchlowerthanforoutbreedingcrossesafteエaboutfour皿on七hsandoneyearintworearinglocalities.Theseresultsindicatetha七inbreedingdepressionisobservedinthedefoエmityandsuLエvivalra七es,butneitherinfertiliza一七ionratenorgrowthinthefirstgenerationofafuU-sibfami1)TofthePacificabalone.Inaddition,wedetectedonemicrosatellitemarkerlinl〈edtoarecessivelethalgeneinaninbreedingfamily,andnowareinvestigatingi七sexistenceino七herfamiliesandthedevθlopmentalstageofitsgeneexpression.ReferencesIkeda,M.,andTaniguGhi,N.Gene七icdiversitアandpopulations七ructureofHanasakicrabIV.In``.Proceeδings〔ゾSαkhNJRO/Nemuo'oJb珈 占IV・rk・勿 か 砺 …`αnath's(鰯 翻 侃P叩 θss伽 ∬y2007αndFuturePlαnofJeintβtu己yon・UαnαsαkiOrαb",CommitteeforNemuroCityHanasakiProgra皿,Nemuro,pp.5-11,2008.Kanne,M.,Suyama,Y.,Li,Q.,andKiji皿a,A・MicrosatelliteanalyFsisofJapaneseseacucumber,S拡c肋興s(オ ア03魏1涙〕μ8)ゴapenicus,supPorbsreproduGtiveisola・tionincolorvariantS・Mα?・ineBtOtechnology,8(6),672-685,2006.Kan-no,M.,andKijima,A,.Gene七icdifferentiationamongthreecolorvariantSofJapaneseseacucumberStdeltOpusゴ(塑oπ魏s.」 醐81紹畑sScience,69(4),806-812,2003.Kan-no,M.andKiji皿a,A.Quantitativeandqualita七iveθvalu-ationon七hecolorvariationoftheJapaneseseacuGumberStdehOpusブ(ゆo舷)us.SUISAハ 「ZOSHO.KU,50(1),63-69,2002.Kijima、,A.CurrentstudiesongenetiesandbreedingsGiencθinaba/one・Jl∠託ni?nalGenetlcs,32(2),101-112,2005.Park,C.,Li,Q.,Kobayashi,T.,andKijima,A・InbreedingdepressiontraitsinPacifiGabaloneHaliotisdiscushαnnαib)rfactOrialmatingexperi.Inents..FisJmaiesSct初nca,72,774-780,2006.Yamashita,M.,KomabSu,M.,andKijima,A.Lowgeneticdifferentiationwiもhhighgeneticvariabilityobservedineommoncoa・sta/star丘shAsterinapaectineferaaroundJapaninferredfromisozymeanalysis.ZInte-grαte{lFieldScience,2,113-121,2005.Yamashita,M.,Ko皿atsu,M.,andKijima,A.Genetievariabilityandgeogエaph-iGalpopulationstructureinGoastals七ar且sh・∠isterilasαmurensisLutkenaroundJapanestimatedbyisozアmeanalysis.FishGenet.Breea.8c盛,34(2),99-109,2005、

Recent Research Topics in Genetic Approaches for Sustainable Yield and Conservation of Marine Organisms

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Recent Research Topics in Genetic Approaches for Sustainable Yield and Conservation of Marine Organisms

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