KANAYAMA  Y.

NARIYAMA  H.

English

NARIYAMA, H.

KANAYAMA, Y.

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Study on the Regulation of Cell Division Potentially Involved in Fruit Size Diversity in Tomato

紀要類 (bulletin)Poster Presentationsdepartmental bulletin pape

Tohoku University Repository (TOUR) / 東北大学機関リポジトリ

Study on the Regulation of Cell DivisionPotentially Involved in Fruit Size Diversityin Tomato著者 NARIYAMA  H., KANAYAMA  Y.journal orpublication titleJournal of Integrated Field Sciencevolume 13page range 47-47year 2016-03URL http://hdl.handle.net/10097/6408547Study on the Regulation of Cell Division Potentially Involved inFruit Size Diversity in TomatoH. NARIYAMA and Y. KANAYAMAGraduate School of Agricultural Science, Tohoku University, JapanPoster PresentationsIntroduction Tomato exhibits genetic diversity in fruit size, shape and color—factors that are useful for breeding. Generally, fruit size depends on cell division and cell expansion, and fruit development consists of a cell division period and a cell expansion period. Therefore, we focused on the regulation mechanism of cell division as one of the determining factors for fruit size. In Arabidopsis roots, HIGH PLOIDY 2 (HPY2) induced by auxin reportedly regulates cell division. In this study, auxin and anti-auxin treatments and the expression analysis of SlHPY2—a tomato homolog gene of HPY2—were performed to reveal the effect of auxin on cell division in tomato fruit.Materials and Methods For auxin and anti-auxin treatment, tomato ‘Ailsa Craig’ fruit was dipped into 1-naphthylacetic acid (NAA) every day from day 4 post anthesis, or into anti-auxin every day from anthesis. Fruit diameter was measured at days 8 and 15 post anthesis. Paraffi n sections prepared from their pericarp were dyed, and the number of cells per unit length in the mesocarp was measured. Total RNA was extracted from the fruit and SlHPY2 expression was measured through quantitative real-time PCR.Results and Discussion In NAA treatment, the number and size of cells did not differ between NAA-treated fruit and the control on day 8 post anthesis, whereas the number of cells was larger and the cell size was smaller in the treated fruit than in the control on day 15 post anthesis. In the expression analysis, SlHPY2 mRNA levels did not differ between NAA-treated fruit and the control on day 8 post anthesis, whereas the levels rose with NAA concentration on day 15 post anthesis. In the anti-auxin treatment, the number of cells was smaller and the cell size was larger in the treated fruit than in the control on both days 8 and 15 post anthesis; however, SlHPY2 mRNA levels did not dif-fer between the anti-auxin treated fruit and the control. These results suggest that auxin promotes cell division, and that further study is necessary to elucidate the role of SlHPY2.

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Study on the Regulation of Cell Division Potentially Involved in Fruit Size Diversity in Tomato

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