Kopahnke, Doris

Ordon, Frank

Pillen, Klaus

Vatter, Thomas

English

JKI Open Journal Systems (Julius Kühn-Institut)

 Vatter et al. 9th Young Scientists Meeting, Quedlinburg, Germany, November 09-11, 2016 Mapping QTL for resistance to net blotch (Pyrenophora teres f. teres) in a wild barley nested association mapping (NAM) popula-tion Thomas Vatter1, Doris Kopahnke1, Klaus Pillen2, Frank Ordon1 1 Julius Kühn-Institut, Institute for Resistance Research and Stress Tolerance, Quedlinburg 2 Chair of Plant Breeding, Institute of Agricultural and Nutritional Sciences, Martin-Luther-University Halle-Wittenberg Email of corresponding author: thomas.vatter@julius-kuehn.de Net blotch, caused by the fungus Pyrenophora teres f. teres, is an im-portant foliar disease of barley causing high yield losses. The identification of QTLs and underlying genes conferring resistance to this fungus is the basis for targeted and sustainable breeding ap-proaches aiming to improve net blotch resistance in modern barley cultivars and to broaden the genetic base of re-sistance. Therefore, a SNP-based nested association mapping (NAM) approach was used to map QTL for resistance de-rived from H. spontaneum or H. agrioc-rithon. To achieve this, the barley nested association population (HEB-25) com-prising 1420 BC1S3 lines in 25 families originating from a cross of 25 wild barley accessions (H. spontaneum and H. agriocrithon) with the cultivar Barkewas screened for resistance in two-yearsfield trials using a summer-hill-design. Best linear unbiased estimates (BLUES) for average ordinate (AO) and reaction type (RT) were calculated. Using these and 5702 informative SNPs obtained from the 9k iSelect barley chip nested association mapping was conducted. Results indicate a high variability in net blotch resistance between and within families of the NAM-population. A high correlation between AO and RT data was observed. In summary, SNPs highly asso-ciated to net blotch resistance were de-tected on all seven chromosomes of which some map to chromosome re-gions previously identified to be linked to net blotch resistance. In a next step, identified QTL will be analyzed in more detail to identify potential candidate genes. Berichte aus dem Julius Kühn-Institut 186 23

Mapping QTL for resistance to net blotch (Pyrenophora teres f. teres) in a wild barley nested association mapping (NAM) population

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Mapping QTL for resistance to net blotch (Pyrenophora teres f. teres) in a wild barley nested association mapping (NAM) population

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