Flachowsky, Henryk

Hanke, Magda-Viola

Miolli, Giulia V.

Si-Ammour, Azeddine

English

JKI Open Journal Systems (Julius Kühn-Institut)

Nachwuchswissenschaftlerforum / Young Scientists Meeting 2011  Giulia V. Miolli et al.    Berichte aus dem Julius Kühn-Institut 162, 2011  Selected apple and strawberry transcription factors and their relevance to fruit development  Giulia V. Miolli1, Henryk Flachowsky2, Madga-Viola Hanke2, Azeddine Si-Ammour1 1Julius Kühn-Institut, Institute for Breeding Research on Horticultural and Fruit Crops 2Fondazione Edmund Mach/CRI, San Michele all'Adige, Italy giulia.molli@jki.bund.de MADS-box transcription factors and their role in flower and fruit development have been widely studied in the model plant Arabidopsis thaliana. However, they are poorly studied in major crop plants and fruit trees. Our study aims to extend the knowledge on MADS-box genes of the Ro-saceae family that includes crops of impor-tant agricultural and commercial value. We selected individual genes of this gene fam-ily involved in developmental processes and studied their function at the molecular level in two Rosaceae representative spe-cies. A phylogenetic analysis of apple MADS-box genes suggests the presence of a subclade containing genes that are close to the Arabidopsis AGL24 gene. Fifteen of these AGL24-like genes were investigated with regard to their function in the apple cultivar 'Golden Delicious'. The ORFs of these genes were validated experimentally from cDNA libraries of different apple tis-sues. Expression studies using qPCR indi-cate that the apple MADS-box genes be-longing to this subclade do not play any role in flower development, but they are more likely involved in response to vernali-zation (dormancy-associated MADS-box, DAM). Furthermore, we studied also the putative F. vesca homologues, but they did not show any expression in the respective tissues of strawberry. Therefore, we fo-cused our study on MADS-box genes with homologues of known function in Arabi-dopsis to perform gene expression and functional analysis. After phylogenetic analysis, three genes that are most likely the strawberry homologues of Arabidopsis PISTILLATA and AGAMOUS were chosen as candidate genes for post-transcriptional gene silencing using a RNA interference approach. The molecular and phenotypic analysis of the transformants will help to explain how the ABCE model genes act in Rosaceae.     18

Selected apple and strawberry transcription factors and their relevance to fruit development

https://ojs.openagrar.de/index.php/BerichteJKI/article/download/1638/1972

Selected apple and strawberry transcription factors and their relevance to fruit development

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