Habekuß, Antje

Ordon, Frank

Perovic, Dragan

Wang, Yaping

English

JKI Open Journal Systems (Julius Kühn-Institut)

10th Young Scientists Meeting, Siebeldingen, Germany, November 08-10, 2017 Wang et al. High-resolution and -density mapping of  Barley mild mosaic virus (BaMMV) resistance gene rym15 Yaping Wang, Antje Habekuß, Dragan Perovic and Frank Ordon Julius Kühn-Institut, Institute for Resistance Research and Stress Tolerance, Quedlinburg E-mail of corresponding author: yaping.wang@julius-kuehn.deBarley is the second most important cereal crop in Europe. Besides others, Barley mild mosaic virus (BaMMV) which is transmitted by the soil-borne protist Polymyxa graminis has a serious impact on barley yield. Although a number of BaMMV resistance genes were already identified, which range from rym1 to rym18, resistance of some genes has been broken by new virus isolates. For example, rym4 is ineffective against BaYMV-2 and rym5 has been shown to be not effective against a new BaMMV strain. Therefore, developing of closely linked molecular markers and next the isolation of up to now less used re-sistance genes is a genuine need for sus-tainable barley production. In previous studies on double haploid (DH) lines derived from the F1 of the cross of the resistant barley accession ‘Chikurin Ibaraki 1’ to the susceptible winter barley cv. ‘Plaisant’ rym15 was located on the short arm of chromo-some 6H. However, the study showed that the order of markers is inverted in relation of the genetic map derived from the cross from ‘Lina’ × Hordeum spontaneum ‘Canada park’. Therefore, our work aims to construct high resolu-tion mapping population of BaMMV re-sistance gene rym15, to (i) resolve the discrepancy between the two maps, (ii) narrow down the target region and satu-rate the map, (iii) with final aim to iso-late rym15. Two crosses derived from resistant bar-ley cv. ‘Chikurin Ibaraki 1’ and suscepti-ble cultivars ‘Uschi’ and ‘Igri’ comprising 2260 and 5671 F2 grains, respectively, will be used for the construction of the high resolution mapping population of rym15. The reaction to BaMMV of ho-mozygous recombinant plants concern-ing the target interval will be assessed by artificial inoculation and DAS-ELISA in green house tests. The Next-generation sequencing (NGS) techniques such as exome capture, and Genotyping by se-quencing (GBS) based bulk segregant analysis (BSA) will be used for the detec-tion of polymorphisms followed by the development of KASPar markers.  Until now, the resistance test of BaMMV in 365 F2 plants originating from ‘Chikurin Ibaraki 1’ × ‘Igri’ cross showed segregation of 85 resistant : 280 suscep-tible. The segregation fitted a ratio 1:3 (χ2=0.571), suggesting the presence of one recessive resistance gene. We geno-typed a set of 365 F2 plants by using 6 SSR markers and 5 KASP markers devel-oped based on the 50K Illumina array data. Genetic map was constructed and new robust co-dominant flanking mark-ers were identified. Our plan is to screen about 5,000 F2 plants with flanking markers, to develop a high density and resolution map of rym15 gene in order to facilitate positional gene isolation. 76 Berichte aus dem Julius Kühn-Institut 192

High-resolution and -density mapping of Barley mild mosaic virus (BaMMV) resistance gene rym15

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High-resolution and -density mapping of Barley mild mosaic virus (BaMMV) resistance gene rym15

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