Fuyuan, X.

Huaying, Z.

Ming, Xu

English

JKI Open Journal Systems (Julius Kühn-Institut)

  IUFRO / REPHRAME International Conference on Pine Wilt Disease 2013  Berichte aus dem Julius Kühn-Institut 169. 2013 86 Huaying Z, Ming Xu, Fuyuan Xu, A comparative proteomics analysis on resistant provenance of Pinus massoniana inoculated with Bursaphelenchus xylophilus. In: Schröder, T. (ed.), Pine Wilt Disease Conference 2013, pp. 86, Braunschweig, ISSN: 1866-590X A comparative proteomics analysis on resistant provenance of Pinus massoniana inoculated with Bursaphelenchus xylophilus Zheng Huaying, Xu Ming, Xu Fuyuan  Forestry Academy of Jiangsu Province, 211153, dongshanqiao, Nanjing, China Email: zhenghy78@aliyun.com ABSTRACT:  Pine wilt disease caused by B. xylophilus, also known as the pine wood nematode (PWN), is the most devastating disease of pine trees. From different geographical provenance of P. massoniana were inoculated with nematodes, test results selected the provenance GD5 which has strongly resistance to PWN. This article used resistant provenance GD5 as the experimental material, and sensitive provenance SX1 as comparison. Total proteins were extracted by using 2-DE and MALDI-TOF/TOF technology from the provenances pine needles respectively. Differentially expressed proteins in the provenance before and two weeks after inoculated with PWN, were analyzed. At last, 89 differentially expression proteins were successfully identified by MALDI-TOF–TOF. The test result also fund that there were five proteins involved in hydrogen peroxide scavenging capacity and protecting the redox homeostasis system from damaged. Their up-regulation may be the main cause of the provenance GD5 resistant to PWN.  Keywords: Pinus massoniana, pine wilt disease, resistant provenance, proteomic, 2-DE, MALDI-TOF/TOF    

A comparative proteomics analysis on resistant provenance of Pinus massoniana inoculated with Bursaphelenchus xylophilus

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A comparative proteomics analysis on resistant provenance of Pinus massoniana inoculated with Bursaphelenchus xylophilus

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