Branchard, M.

Netzer, M. H.

English

JKI Open Journal Systems (Julius Kühn-Institut)

466 Section 5 Vitis sp.: Somatic embryos obtained on medium inducing calcareous chlorosis M.H. NnzER and M. BRAl'CHARD L.A.P., UA 115, Bt 360, Universire de Paris-Sud, F-91405 Orsay Cedex, France A b s t r a c t : Somatic embryogenesis is one of the techniques used to screen for plants resistant to calcareous chlorosis. In the Plant Breeding Labaratory in Orsay, LEBRUN and BRANCHARD (1987) have obtained somatic embryos for different genotypes. Also, CHtADMI and BRANCHARD (1985) defined ferric and bicarbonic ions which are able to induce a resistance to calcareous chlorosis. Anthers of diverse grapevine rootstocks, susceptible (Riparia Gloire 1 Rupestris du Lot 1 3 309 Couderc) and resistant (Fercal 1 41 B) to calcareous chlorosis were cultivated in chlorosis inducing medium with increasing concentration offerric sulfat and ofpotassium bicarbonate (from 50 to 1000 mg/ 1). For each replicate, 30 sterilized tlowers were dissecred and starnens were cultivated in an Erlenmeyer tlask and maintained at 28-30 °( ± 1 °( on a rotary shaker in the dark for one month. At present, the results observed in chlorosis medium indicate an imponant stamen necrose at high concentration. However, callus appearance is delayed when low concentrations are used. The ftrst somatic embryos have appeared and have begun regenerating. Grapevine shoot formation in vitro J. K."ROGL.-\:-;, !'\. MtROSE\K and S. JELASKA Faculty of Agriculture, Institute ofFruit Trees, Grapes , Oenology and Honiculture, Kaciceva 9, YU-41000 Zagreb, Yugoslavia Abstract : In studies on in virro propagation of Viris vinifera L. (cvs Chardonnay, Pinot white, Sultanina. Plavac mali and Plavac mali sivi, a spontaneaus variant), shoot explants, 1·2 mm long, were grown on media with different types and concentrations of growth regulators, macronutrients and carbohydrates. Excised buds from canes: collected in March and held in warer andin a controlled Iabaratory environment, were the source of explant~. Very successful surface sterilization ofbuds was achieved with 1.5% Izosan (chlorine product, Pliva, Zagreb) and 0.01 % Tween 20 for 15 min, and rinsed in sterile distilled water (3 x 5 min) . Two basal media with macronutrients according to Ml:R.-\SHIGE and SKOOG (MS), full and halfstrength. and LLOYD and McCo"~ (WPM) supplemented with 2% sucrose, 0.8% Difco Bacto agar and (mgJ· 1): 1 thiamine: HCI, 0.5 pyridoxine: HCL 2 glycine. 100 myo-inositol were tested. Cytokinin BA and auxins lA-'\ and ?\A.A in different concentrations and combinations were studied. The full strength MS medium with 1 mgJ· 1 BA and 0.3 mgJ· 1 lA-'\ gave the best results in establishing multiple shoot cultures. Elfecrs of cultivar and time length in culture on multiplication rate, leaf shape de\iation and vitriftcation was analysed. 

Vitis sp.: Somatic embryos obtained on medium inducing calcareous chlorosis

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Vitis sp.: Somatic embryos obtained on medium inducing calcareous chlorosis

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