Cao, Zi Yi

Guo, Cai Yue

Li, Wei

Qi, Yu Shu

English

JKI Open Journal Systems (Julius Kühn-Institut)

Tissue and cell culture Embryogenie cell lines from somatic embryos of grape ( Vitis Yinifera L.) K MATSUTAand T. HIRABAYASHI Fruit Tree Research Station, Tsukuba, Ibaraki 305, Japan 467 Ab s t r a c t : Somatic embryo fonnation occuiTed on leaf callus of grape ( Vitis l'inifera cv. Koshusanjaku). i\n embryogenic callus was induced from somatic embryo clusters cultured on vitamine-, inositol- and glycine-free l\nsc:H and ?\:nsc.H (1969) medium supplememed with 1.011 M 2,4-D. This callus has retained a high embryogenic activity after repeated subculture on the same medium for over 2 years and has produced numerous embryos after transfer to a honnone-free medium. The effect of cytokinin treatment on somatic embryogenesis from leaf callus was also examined . l\:-(2-chloro-4-pyridyl)-l\"-phenylurea (KT-30) and N-(1,2,3-thiadiazol-5-yl)-l\"-phenylurea (TAG), both synthetic cytokinins, were found to be effective for the induction of somatic embryogenesis. When leafcallus was induced by these cytokinins combined \vith 2,4-D at either 5.0 or 10.0 11M, somatic embryos were produced. The regeneration of plantlets from culture anthers picked from anther culture derived plants in grape in Yitro Z1 Y1 C>.o, WEJ L1, C."I YeE GL:o and Yv Sm: Qr Gansu Agricultural Universiry, Wuwei, Gansu, China Abstract : The induction of plantlets by grape amher culture is difflcult because only a few genotypes can induce plant Iets from amhers and the frequency of plant regeneration is very low. The goal of the presem study is to detennine if the frequency of plantlet regeneration can be increased by the culture of anthers picked from the anther culture derived plants as it does in cereal crops. The grape inflorescences ofV70 line, a line derived from the amher culture, which werein uninucleate po!len stage and the flower buds just separated, were inoculated on the B5 medium supplemented with 0.5 ppm 2,4-D, 2 ppm BA, 3% sucrose, and cultured under 28 ac :1:2 °C for 1-1.5 months, and then transferred on regenerationmedium (B5 supplemented with 4 ppm BAP and 0.2 ppm KAA). After 4 months, the embryoids regenerated from the calli of anthers, and plantlets fonned when the embryoids were transferred onto shoot regeneration medium. The results showed that the frequency ofplantlet regeneration from anthers picked from anther culture derived plants is high er than that from amhers picked from the stock plams. Thus, crosses between genotypes which can be induced to fonn plants from anther culture and others which cannot will possibly increase the inducible materials. 

The regeneration of plantlets from culture anthers picked from anther culture derived plants in grape in vitro

https://ojs.openagrar.de/index.php/VITIS/article/download/5551/5310

The regeneration of plantlets from culture anthers picked from anther culture derived plants in grape in vitro

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