Fazlikhani, Leila

Kopahnke, Doris

Ordon, Frank

Perovic, Dragan

English

JKI Open Journal Systems (Julius Kühn-Institut)

10th Young Scientists Meeting, Siebeldingen, Germany, November 08-10, 2017 Fazlikhani et al. Marker saturation of the RphMBR1012 locus confering resistance against Puccinia hordei in barley using the 50K iSelect chip and Geno-typing by Sequencing (GBS)  Leila Fazlikhani, Dragan Perovic, Doris Kopahnke and Frank Ordon Julius Kühn-Institut, Institute for Resistance Research and Stress Tolerance, Quedlinburg E-mail of corresponding author: leila.fazlikhani@julius-kuehn.deRecent advances in the development of barley genomic resources i.e. 9K and 50K iSelect arrays, genome zipper, POPSEQ, and GBS maps, as well as the barley ref-erence sequence facilitate enhanced identification of resistance genes. The resistance gene RphMBR1012 previously mapped on the short arm of barley chromosome 1H is effective against the highly virulent barley leaf rust (Puccinia hordei) isolate I-80. In order to isolateRphMBR1012 using a map-based-cloning approach, marker saturation of the tar-get region and construction of a high resolution mapping population were undertaken in parallel based on the cross “MBR1012 (resistant) x Scarlett (susceptible)”. 492 segmental homozy-gous recombinant inbred lines (RILs)derived from 4775 F2-plants were identi-fied by analyzing the population with two co-dominant flanking markers, sep-arated by 8.0 cM. For marker saturation 35 SSRs and SNP markers from the ge-nome zipper, and 9K iSelect chip con-verted to PCR-based markers, were used. Using this approach the target interval was shortened to 0.15 cM. Using data from the 50k iSelect chip and geno-typing by sequencing 19 additional markers were identified and the target interval was further delimited to 0.095 cM. By blasting these markers to the reference sequence, the physical size of the interval was determined at 0.4 Mb. Five disease resistance like genes were identified in the target interval and are now re-sequenced. 26 Berichte aus dem Julius Kühn-Institut 192

Marker saturation of the RphMBR1012 locus confering resistance against Puccinia hordei in barley using the 50K iSelect chip and Genotyping by Sequencing (GBS)

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Marker saturation of the RphMBR1012 locus confering resistance against Puccinia hordei in barley using the 50K iSelect chip and Genotyping by Sequencing (GBS)

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