Briem, Felix

Eben, Astrid

Staudacher, Karin

Traugott, Michael

Vogt, Heidrun

Zeisler, Christian

English

JKI Open Journal Systems (Julius Kühn-Institut)

 Briem et al. 9th Young Scientists Meeting, Quedlinburg, Germany, November 09-11, 2016 Landscape-level movements and a molecular approach to analyze the diet of Drosophila suzukii  Felix Briem1, Karin Staudacher2, Christiane Zeisler², Astrid Eben1, Michael Traugott² and Heidrun Vogt1 1 Julius Kühn-Institut, Institute for Plant Protection in fruit Crops and Viticulture, Dossenheim 2 University of Innsbruck, Institute of Ecology, Innsbruck, Austria Email of corresponding author: Felix.Briem@julius-kuehn.de The invasive pest species Spotted Wing Drosophila (SWD), Drosophila suzukii, has been first recorded in the USA and Southern Europe in 2008. Since 2011 it is also known from Germany. D. suzukii is an extremely polyphagous pest species. It feeds and reproduces on more than 120 cultivated and wild fruits from 20 plant families. Infested fruits quickly collapse and become unmarketable. D. suzukii overwinters as adult in a repro-ductive diapause at sheltered sites, es-pecially in forests and hedges. Since this invasive pest is active at mild days in winter and spring it needs nutritional resources.  To investigate which resources are used, monitoring adult activity, laboratory bioassays and molecular techniques were applied to identify wild host plants sustaining D. suzukii during winter and spring. The occurrence of D. suzukii at landscape level and its remigration into orchards was observed all year round. Further, an automatic trap was devel-oped to examine the diurnal activity of D. suzukii. No-choice assays with mistle-toe berries were established to investi-gate egg laying, feeding activity and sur-vival of D. suzukii. For investigating theattraction of D. suzukii to trees parasi-tized with mistletoe, Viscum album, weanalyzed the volatile organic compounds(VOCs) of berries by GC–MS. Feedingexperiments (FEX) were established toexamine the digestion time and to de-velop a molecular approach to identifyfood sources during winter and spring. Additionally, bleaching experiments (BEX) were conducted in order to decon-taminate the body surface which should not negatively affect the DNA of the gut content.  During fall and early winter, catches in monitoring traps indicated a shift of fly activity from orchards towards forests and hedges. Significantly higher num-bers of flies were captured in the canopy of Pinus sylvestris parasitized with V. album compared to non-parasitized trees. From April onwards we found fe-males with mature eggs coinciding with ripe berries of V. album. Under laborato-ry conditions eight adult individuals emerged from 1.100 field-collected ber-ries in 2015. The odor spectrum identi-fied from the berries was comparable to common berry odors. Further, we suc-ceeded in identifying ingested chloro-plast-DNA in the gut content D. suzukii. First results of the FEX show that fe-males ingest more DNA than males. Fol-lowing, the results of the BEX show that the body surface was decontaminated whereas the chloroplast DNA of the gut content stays unaffected.  The combination of the presented field studies and laboratory assays will pro-vide important information to identify the nutritional resources of D. suzukii in winter and spring, and to get a better understanding of D. suzukii behavior on host plants in the course of a day.  Berichte aus dem Julius Kühn-Institut 186 13

Landscape-level movements and a molecular approach to analyze the diet of Drosophila suzukii

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Landscape-level movements and a molecular approach to analyze the diet of Drosophila suzukii

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