Hausmann, Ludger

Holtgräwe, Daniela

Kamal, Nadia

Ochßner, Iris

Rosleff Sörensen, Thomas

Töpfer, Reinhard

Weisshaar, Bernd

Werner, Anna

English

JKI Open Journal Systems (Julius Kühn-Institut)

Nachwuchswissenschaftlerforum / Young Scientists Meeting 2015 Werner et al. Genetics of flowering time in grapevine Anna Werner1, Iris Ochßner1, Ludger Hausmann1, Nadia Kamal2, Daniela Holtgräwe2, Thomas Rosleff Sörensen2, Bernd Weisshaar2, Reinhard Töpfer1 1 Julius Kühn-Institut, Institute for Grapevine Breeding Geilweilerhof, Siebeldingen 2 Bielefeld University, Faculty of Biology, Center for BiotechnologyEmail of corresponding author: anna.werner@jki.bund.de Due to climatic change, phenology traits are becoming increasingly important in grapevine breeding, since a premature flowering and ripening time could be ob-served for grapevine in the last decades. However, knowledge about these traits is still limited as they are genetically very complex and highly influenced by envi-ronmental factors. The analysis of the ge-netic basis of flowering time therefore will enable the development of tightly linked molecular markers useful for marker-assisted selection of especially late flower-ing breeding lines. A QTL analysis approach was followed to detect major genetic factors within a biparental mapping population derived from a cross of the early-flowering breed-ing line GF.GA-47-42 and late-flowering cultivar ‘Villard blanc’. Phenotyping of flowering time was carried out for the 150 F1 individuals in seven years. First investi-gations indicate QTLs on six chromosomes, some of which could also be confirmed in a second unrelated mapping population. Analysis of the QTLs indicates that certain combinations of marker alleles from thedifferent QTL regions lead to a very early, medium or late flowering phenotype, re-spectively, and suggests a dominant effect for early flowering. However, the revealed QTLs still cover large regions of the re-spective chromosome which hampers the detection of credible candidate genes. Future aims of the project are: (1) Fine mapping of the QTL regions by developing new SSR markers and also with genotyp-ing-by-sequencing (GBS) of a subset of F1 individuals. Additionally, an extended mapping population of approximately 900 F1 plants is available for phenotyping, in order to refine the QTL regions, hence limit the number of candidate genes and determine more reliable ones. (2) A deep-er investigation of allelic variation at the QTL regions and the impact of their com-bination on the flowering time phenotype. (3) Flowering time homologues described in model plants and located within a QTL are examined for their expression at vari-ous developmental time points in both parents to find differences between the early and late genotype. Berichte aus dem Julius Kühn-Institut 181 39

Genetics of flowering time in grapevine

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Genetics of flowering time in grapevine

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