Baranska, Malgorzata

Baranski, Rafal

Dudek, Monika

Grzebelus, Ewa

Kaczor, Agnieszka

Machalska, Ewa

Oleszkiewicz, Tomasz

Pacia, Marta Z.

Rygula, Anna

Zając, Grzegorz

English

JKI Open Journal Systems (Julius Kühn-Institut)

2nd International Plant Spectroscopy Conference (IPSC) 2019, Berlin, 24 - 28 March 38 03-02: Raman spectroscopy combined with AFM reveals complexity of carotenoid samples Malgorzata Baranska1, Anna Rygula1, Marta Z. Pacia1, Monika Dudek1, Ewa Machalska1, Grzegorz Zając1, Agnieszka Kaczor1, Tomasz Oleszkiewicz2, Ewa Grzebelus2, Rafal Baranski2 1Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; E-mail: baranska@chemia.uj.edu.pl 2Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland The paper shows a potential of Raman spectroscopy for analysis of carotenoids in a form of crystals and in the solution as aggregates. Spectroscopic measurements of carotenoid crystals are combined with Atomic Force Microscopy (AFM) and Scanning Near-Field Optical Microscopy (SNOM), whereas aggregates are investigated with the use of Raman Optical Activity (ROA) spectroscopy. Spectroscopic and microscopic scanning probe measurements were applied to the released crystals or to crystals accumulated in a unique, carotenoids rich callus tissue growing in vitro. Three distinct morphological crystal types of various carotenoid composition were identified, a needle-like, rhomboidal and helical. Raman imaging provided evidence that the needle-like and rhomboidal crystals had similar carotenoid composition and that they were composed mainly of β-carotene accompanied by α-carotene. AFM measurements of crystals revealed the crystal topography and showed the needle-like and rhomboidal crystals were planar but they differed in all three dimensions. Combining SNOM and Raman imaging enabled indication of carotenoid rich structures and visualised their distribution in the cell.  In the second part, a stereochemistry of carotenoids is investigated. Carotenoids dissolved in organic-water media can form two types of aggregates: H (card-packed) and J (head-to-tail) that exhibit hypsochromic and batochromic shift of chromophore absorption, respectively. With the help of (resonance) ROA spectroscopy detailed information about the structure and configuration of chiral, supramolecular carotenoid assemblies is obtained. References  [1] RYGULA, R., OLESZKIEWICZ, T., GRZEBELUS, E., PACIA, M.Z., BARANSKA, M., and R. BARANSKI, 2018: Spectrochimica Acta A, 197, 47-552. 

Raman spectroscopy combined with AFM reveals complexity of carotenoid samples

https://ojs.openagrar.de/index.php/BerichteJKI/article/download/11201/10170

Raman spectroscopy combined with AFM reveals complexity of carotenoid samples

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