Ruge-Wehling, Brigitte

von Hülsen, Behrend

English

JKI Open Journal Systems (Julius Kühn-Institut)

Nachwuchswissenschaftlerforum / Young Scientists Meeting 2014 von Hülsen & Ruge-Wehling    DURESTrit - mapping NHR-genes from barley secondary gene pool Hordeum bulbosum  Behrend v. Hülsen1, Brigitte Ruge-Wehling1 1 Julius Kühn-Institut, Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz Email of corresponding author: behrend.huelsen@jki.bund.de  As part of the ERA-CAPS program the DURESTrit consortium is an interna-tional collaboration between the Julius Kühn-Institut, the Leibniz Institute of Plant Genetics and Crop Plant Research and several national and international partners. Aim is the localization and functional characterization of nonhost resistances from the primary and sec-ondary gene pool in barley. Nonhost Resistance (NHR) is the re-sistance plants possess against the ma-jority of pathogens in the environment. Therefore transferring components of NHR to crop plants from related species via introgression lines will give us new tools to protect our crops against path-ogens. Our part of the project is to map new powdery mildew resistance loci for Bar-ley, derived from the secondary gene pool Hordeum bulbosum. We use sev-eral 2HS and 2HL introgression lines differing in their H. bulbosum and Hordeum vulgare backround. All intro-gressions bear at least two different resistances against Blumeria graminis f. sp. hordei.           For molecular characterization a map-ping population will be developed. Phenotyping will be carried out by de-tached leaf assays with different pow-dery mildew isolates, checking the spectrum of resistance.  Fine Mapping of the introgressions will be performed by novel marker tech-niques, e.g. Exome Capture and GBS. Based on the resulting sequence infor-mation CAPS, InDel, as well as SNP marker will be constructed for marker assisted selection of the novel re-sistance loci. Additional transcript in-formation can be used as candidate genes getting closer to resistance loci. Recombinant plants with reduced in-trogression sizes will be selected with minimized introgression size and link-age drag to increase the value for breeding purposes.  Berichte aus dem Julius Kühn-Institut 177 47

DURESTrit - mapping NHR-genes from barley secondary gene pool Hordeum bulbosum

https://ojs.openagrar.de/index.php/BerichteJKI/article/download/3512/3581

DURESTrit - mapping NHR-genes from barley secondary gene pool Hordeum bulbosum

Abstract

Similar works

Full text

Available Versions

JKI Open Journal Systems (Julius Kühn-Institut)