Negisho, Kefyalew

Ordon, Frank

Teklemariam, Surafel

Wehner, Gwendolin

English

JKI Open Journal Systems (Julius Kühn-Institut)

11th Young Scientists Meeting 2018, Braunschweig, Germany, November 14-16 Teklemariam et al. Evaluation of Ethiopian barley landraces for drought stress tolerance  Surafel Teklemariam 1,2, Kefyalew Negisho2,3, Gwendolin Wehner2 and Frank Ordon2 1Ethiopian Institute of Agricultural Research (EIAR), Melkassa Agricultural Research Center, Melkassa, Ethiopia  2Julius Kühn-Institut, Institute for Resistance Research and Stress Tolerance, Quedlinburg  3EIAR, National Agricultural Biotechnology Research Center, Holetta, Ethiopia E-mail of corresponding author: surafel.teklemariam@julius-kuehn.deDrought is the main limiting factor for yield losses in Ethiopia. Barley (Hordeum vulgare L.) is the best adapted cereal crop in Ethiopia which is grown from drought-prone areas of an altitude of 1500 meter above sea level up to the highlands of Ethiopia (3400 m) which are character-ized by a temperate climate with ade-quate rainfall. Thus, barley landraces from Ethiopia may be a valuable source to exploit drought stress tolerance. To achieve this, field experiments including 260 Ethiopian barley landraces were con-ducted in two cropping seasons in Ethio-pia (2016 and 2017) at two locations with natural drought stress (Melkassa and Dera) and two locations having adequate moisture conditions (Holetta and Debre Zeit). Additionally, landraces were geno-typed with the 50k iSelect chip to identify genomic regions involved in drought stress tolerance by genome wide associ-ation studies (GWAS). The cropping data of 2017 was omitted from further analysis due to the presence of adequate rainfall in drought stress lo-cations. Analysis of variances (ANOVA) revealed significant (p<0.001) differences between control and drought stress con-ditions for total grain yield in 2016. Fur-thermore, significant (p<0.001) effects between the landraces were observed, representing high genotypic variation. Total grain yield at drought stress condi-tions (Ys) was significantly (p<0.001) cor-related with days to flowering (r=-0.68) and maturity (r=-0.42)  Drought indices like stress tolerance in-dex (STI) and stress non-stress produc-tion index (SNPI) were used to cluster the landraces, by tolerance to drought stress. Thus, e.g. landraces B191.1 and B222 were clustered as best performing ones under drought stress.  One year field experiments revealed gen-otypic variation in Ethiopian barley land-races. In a next step, genome wide asso-ciation studies will be conducted in order to identify genomic regions involved in drought stress tolerance and to develop molecular markers suited to be used in future barley breeding.Berichte aus dem Julius Kühn-Institut 200 25

Evaluation of Ethiopian barley landraces for drought stress tolerance

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Evaluation of Ethiopian barley landraces for drought stress tolerance

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