Flachowsky, H.

Hanke, M.-V.

Wenzel, S.

English

JKI Open Journal Systems (Julius Kühn-Institut)

Nachwuchswissenschaftlerforum / Young Scientists Meeting 2012 Wenzel et al. 49 Berichte aus dem Julius Kühn-Institut 167. 2012  Heat shock induced flowering of PtFT apple plants Wenzel, S.1, Flachowsky, H.1, Hanke, M.-V.1   1 Julius Kühn-Institute, Institute for Breeding Research on Horticultural and Fruit Crops, Dresden-Pillnitz Email of corresponding author: viola.hanke@jki.bund.de  Apple plants have a long vegetative phase of 7 to 10 years involving a time consuming, non-economical process of breeding apple resistant to fire blight, scab and powdery mildew. An early onset of flowers would be a great advantage to release disease resistant pre-breeding material. Over-expression of the flower promoting genes LEAFY and FLOWERING LOCUS T from Arabidopsis thaliana in poplar (Populus trichocarpa) and BpMADS4 from Betula pendula in apple (Malus × domestica) led to an accelerated development of flowers. Over-expression of the BpMADS4 gene in apple resulted in constitutive flowering from in vitro culture to greenhouse. A continuous develop-ment of fruits was achieved based on the constitutive expression of the 35S promoter in these plants. This extensive fruit production resulted in an abnormal vegetative plant development, in small-sized fruits and in high fruit drop due to a lack in nutrient supply. As an improved approach a heat-induced flowering was established based on the heat-shock promoter Gmhsp 17.5-E (HSP) from soybean (Glycine max). In poplar a heat-regulated expression of the poplar FLOWERING LOCUS T (PtFT) gene resulted in flowering after certain heat-treatments. We introduced the PtFT1 and PtFT2 gene from poplar under the HSP-promoter into apple. The conditions for heat-induced regulation of these genes in apple were studied.  

Heat shock induced flowering of PtFT apple plants

https://ojs.openagrar.de/index.php/BerichteJKI/article/download/2071/2451

Heat shock induced flowering of PtFT apple plants

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