Bziuk, Nina

Fornefeld, Eva

Nour, Eman

Smalla, Kornelia

English

JKI Open Journal Systems (Julius Kühn-Institut)

 9th Young Scientists Meeting, Quedlinburg, Germany, November 09-11, 2016 Bziuk et al. Enrichment of IncP-1 plasmid carrying bacte-ria in the rhizosphere of lettuce and tomato – is there a fitness advantage? Nina Bziuk, Eva Fornefeld, Eman Nour, Kornelia Smalla Julius Kühn-Institut, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig Email of corresponding author: nina.bziuk@julius-kuehn.de Plasmids can offer bacteria a wealth of accessory functions enabling the survival and adaptation of a population in heter-ogeneous conditions or under environ-mental stresses. IncP-1 plasmids belong to the group of broad host range plas-mids which are of great interest due to their ability to shuttle between different members of bacterial communities un-der various conditions. Furthermore, the plasmids can replicate in a wide range of hosts. This plasmid group was detected in different environments such as sew-age, soils and river sediments and its abundance seemed to be correlated with pollutants. They have a conserved plasmid backbone containing e.g. the korB gene which is specific for this plas-mid group and is used for the detection of IncP-1 plasmids. The backbone con-tains also hot spots of insertion where accessory elements can be inserted. IncP-1 plasmids can carry multiple anti-biotic resistances and could easily enter the food chain via plant-associated bac-teria. An enrichment of IncP-1 plasmids in soil is often found in response to pollution, like the application of manure contain-ing antibiotics. Recently, an enrichment of IncP-1 plasmids could also be shown in the rhizosphere of lettuce plants grown in untreated soil. The result based on the quantification of korB in total community DNA and was compared to bulk soil. The enrichment of IncP-1 plasmids in the rhizosphere compared to bulk soil is plant species dependent which could be shown in another study investigating the rhizosphere of tomato and potato. The abundance of IncP-1 plasmids was higher in the rhizosphere of tomato compared to bulk soil, but not in potato rhizosphere.  To further investigate the unexpected high abundance in the lettuce rhizosphere, IncP-1 plasmids were cap-tured from rhizosphere bacteria in a triparental mating using their ability to mobilize an IncQ plasmid. The majority of the transconjugants obtained be-longed to the IncP-1β subgroup.  In current experiments, competition experiments are performed to analyze the potential fitness advantage of Pseu-domonas putida in the rhizosphere given by the plasmids. Therefore, different P. putida transconjugants are inoculatedtogether with a non-plasmid bearingstrain in a 50/50 ratio into DS soil withlettuce and tomato plants. Rhizospheresamples are taken using the Stomachermethod by which the soil directly attach-ing the root can be washed off. Thesamples are used for CFU counts andtotal community DNA extraction. Quan-tification of korB is done by taqmanqPCR to analyze a potential enrichmentin non-inoculated plants. First resultswill be presented at the conference.Berichte aus dem Julius Kühn-Institut 186 43

Enrichment of IncP-1 plasmid carrying bacteria in the rhizosphere of lettuce and tomato – is there a fitness advantage?

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Enrichment of IncP-1 plasmid carrying bacteria in the rhizosphere of lettuce and tomato – is there a fitness advantage?

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