Hallmann, Johannes

Heuer, Holger

Topalovic, Oliveira

English

JKI Open Journal Systems (Julius Kühn-Institut)

 Topalovic et al. 9th Young Scientists Meeting, Quedlinburg, Germany, November 09-11, 2016 Suppression of infective stages of phytonematodes by associated microbiomes Olivera Topalovic1, Johannes Hallmann2, Holger Heuer1 1 Julius Kühn-Institut, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig 2 Julius Kühn-Institut, Institute for Epidemiology and Pathogen Diagnostics, Münster Email of corresponding author: olivera.topalovic@julius-kuehn.de The role of soil-borne microbiomes in suppression of plant diseases caused by plant-parasitic nematodes (PPN) has become a focus of many studies and search for putative microbial suppres-sors is essential in this regard. Our pro-ject is based on the assumption that specific microbes attach to migrating infective stages of PPN in soil and trigger induced systemic resistance in plants, rather than directly antagonizing nema-todes. To better understand this paradigm of nematode-plant-microbe interactions we have designed a greenhouse experi-ment where we tested three different soils for their suppression against root-knot nematode Meloidogyne hapla. In-fective second-stage juveniles (J2) of M. hapla were baited in soil suspensionof respective soils and inoculated in thepots with tomato plants. After one weekwe stained the roots and found a signifi-cant reduction in number of invading J2in two soils compared to the control,and a reproduction rate is yet to be de-termined. In order to detect and isolatemicrobes that are responsible for thissuppression, we aim to apply culture-dependent and culture-independentmethods, including the next generationsequencing.As hypothesized that soil microbes spe-cifically attached to J2 induce systemic resistance in plants, we established pro-tocols for RNA extraction from roots and RT-PCR to analyse the expression of de-fence genes. In this case surface-sterilized nematodes are baited in soil suspensions and applied to aseptic to-mato plants. In addition, we have suc-ceeded to produce aseptic tomato plants via callus in order to avoid the interference of nematode-attached mi-crobes with plant endophytes when as-sessing the induced systemic resistance in plants. Relying on our work to date, we are aim-ing to expand our study and include some other nematode species and dif-ferent populations of M. hapla so we could see if the same story applies to all of them and/or which differences are involved.  Biological control of PPN has gained an important place in successful agriculture systems and our aim to widen knowledge in nematode suppression by specifically attached soil microbes will be the basis for including a soil micro-biome management in integrated con-trol strategies. 26 Berichte aus dem Julius Kühn-Institut 186

Suppression of infective stages of phytonematodes by associated microbiomes

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Suppression of infective stages of phytonematodes by associated microbiomes

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