Ampelometric studies on 26 varieties of Vitis vinifera L. belonging to the germplasm existing in the collection of Tighennif (Mascara), the most important conservatory of local grapevine varieties existing in Algeria were carried out to characterize this gene pool, the phyllometric measurement method proposed by MARTÍNEZ and GRENAN was applied to establish a cultivar specific adult leaf. Statistical analysis was performed to identify the most discriminating parameters, namely, size of angles and depth of the lateral sinuses in comparison to the lengths of the veins, especially those on the left side of the leaf. Thus, cultivars with common features such as 'Bezoul el Khadem' and 'Ahmar de Mechtras III', 'Toutrissine' and 'Aberkane' and 'Amellal' and 'Torki' were clustered together. For seven varieties the average leaf has been reconstructed.