Kollar, Andreas

Porsche, Franziska M.

English

JKI Open Journal Systems (Julius Kühn-Institut)

Nachwuchswissenschaftlerforum / Young Scientists Meeting 2013 Porsche & Kollar     Saponins from Sapindus mukorossi:  Extraction, purification and biological activity against apple scab   Franziska M. Porsche and Andreas Kollar Julius Kühn-Institut, Institute for Plant Protection in Fruit Crops and Viticulture, Dossenheim Email of corresponding author: franziska.porsche@jki.bund.de  Saponins from soap nut, Sapindus mukorossi, show high antifungal activity against the plant pathogen Venturia inaequalis. In seedling assays, a treat-ment with S. mukorossi extract (1%) reduced apple scab symptoms. Patho-gen sporulation on infected leaves was reduced (99%) as compared to control.   New extraction and purification meth-ods to analyze and characterize the antifungal saponins from S. mukorossi were developed. Fine-grinded pericarps of Sapindus mukorossi were extracted with a chloroform-methanol-water mix-ture. In a next step chloroform and wa-ter was added to achieve phase separa-tion. The supernatant was removed and lyophilized. Further purification was achieved by hydrophobic interaction preparative column chromatography.For the first time Phenyl Sepharose High Perfomance was used for saponin separation.  Detection of membranolytic/hemolytic fractions was performed with an agar diffusion assay amended with sheep erythrocytes. Fractions were further purified and analyzed with TLC. Sepa-rated bands were visualized, scraped off and extracted with 80%. ethanol. These compounds were further charac-terized by HPLC analyses. Peaks were fractionated and subjected to bioas-says. One fraction showed significant fungizidal effect on conidia germination and growth of Venturia inaequalis.   42 Berichte aus dem Julius Kühn-Institut 171

Saponins from Sapindus mukorossi: Extraction, purification and biological activity against apple scab

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Saponins from Sapindus mukorossi: Extraction, purification and biological activity against apple scab

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