Kassemeyer, H.-H.

Staudt, G.

English

JKI Open Journal Systems (Julius Kühn-Institut)

Tissue and cell culture 465 Elimination of virus diseases by in vitro culture G. STAUDTand H.-H. K..>.ssEMEYER Staatliches Weinbauin.stitut Freiburg, Merzhauser Str. 119, D· 7800 Freiburg, F. R. Germany Ab s t r a c r : Arabis mosaic Yirus (ArMV) and raspben·y ringspol virus (RRV) are causal agems of grapevine fanleaf disease, one of rhe most damaging virus diseases of grapevines occuning in Gennany. A simple method has been developed ro eliminate the viruses using in vilro propagarion ofsingle nodes. ?>iodes from ArMV or RRV-infecred vines were micropropagared 10 complere plams and cultivared in a modif1ed Murashige-Skoog medium. The posirion of individual nodes on their morher plant was documenred. For any new subculture, rhe plants were dissecred to single nodes when they reached a cenain lengrh. These were cultivared separarely. After three in I'Üro sub-culrurings, rhe plants were transferred ro pors with a substrate comaining a disinfecred loess/sand mi..xture and culrivared under greenhause conditions. After each in vilro subculture and 18 mönths afrer rhe beginning of rhe in l'itro culrure, the resulting plams were resred using ELISA for rhe presence of ArMV and RRV, respectively. After the first and second subculture, a cenain number of plants already showed no detectable viruses. After 18 months, no con·eiation could be detected berween the origin of the plant from any panicular node and virus elimination. The results suggesr thar this in virro culture merhod makes elimination of ArMY and RRV possible wirhin 18 monrhs wirhaut any thennotherapy. Agrobacterium-mediated transformation of grapevine ( Vitis vinifera L.) V. Gt ;ELLEC 1). C. DAnD, A. PETIT, J. TE'.IPEand M. 8R..>.NCHARD 1) L.A.P .. UA 115, Bt 360, Universire de Paris-Sud, F-91405 Orsay Cedex, France A b s r r a c r : Genetically rransfom1ed grapevine ( Viris vin!(era L.) roots were obtained after inoculation of in virro grown whole plants (cv. Grenache) wirh Agrobacrerium. The strain used comains two independant plasmids: the wild type Ri-plasmid pRi 15834 (agropine type) and a Ti-derived plasmid which carries the neomycin phosphotransferase II gene (:\TPTII) and the nopaline synthase gene. Expression ofrhe :\PTII gene can confer kanamycin resistance to transfonned plant cells. Axenic root cultures derived from single root tips were obtained. Opine analysis indicated the presence of agropine and/or nopaline in established root cultures. For one of the cultures the presence of Ri and Ti derived D:\'A was confmned by Dot-blot hybiidizations of genomic root D:'\A with pRi 15834 TL-D:\'A and the :\'PT II gene. Callogenesis was induced by subculturing root fragments on MuR..>.SHJGE and SKooG (1962) medium supplemented with benzylamino purine (0.2 mg/1) and indoleacetic acid (0.5 mg/1). 

Elimination of virus disease by in vitro culture

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Elimination of virus disease by in vitro culture

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