Research NoteThe occurrence of another natural mutation in cv. Italia is described, which is the main table grape variety cultivated in Brazil. The mutant cultivar from unknown origin is named Redimeire in its growing region. Its fruits are long-ovally shaped, with medium length/width ratio of 1.73, purple coloration and delicate muscatel taste

Botelho, R. V.

Conagim, A.

Nogueira, N. A. M.

Pires, E. J. P.

Sawazaki, H. E.

Terra, M. M.

English

JKI Open Journal Systems (Julius Kühn-Institut)

Vitis 42 (1), 55–56 (2003)Research NoteRedimeire: A natural mutation ofcv. Italia in BrazilE. J. P. PIRES1) , H. E.  SAWAZAKI1), M. M. TERRA1), R. V.BOTELHO1), A. CONAGIM1) and N. A. M. NOGUEIRA2)1) Instituto Agronômico (IAC), Campinas, Brazil2) Coordenadoria de Assistência Técnica Integral, Jales, BrazilSummary: The occurrence of another natural muta-tion in cv. Italia is described, which is the main table grapevariety cultivated in Brazil. The mutant cultivar from un-known origin is named Redimeire in its growing region.Its fruits are long-ovally shaped, with medium length/widthratio of 1.73, purple coloration and delicate muscatel taste.K e y   w o r d s :  Vitis vinifera, new variety, genetics, RAPD.Introduction: Mutations are the base for genetic selec-tion in a plant population. In general, they are accidents thathappen in a fortuitous and unpredictable manner, with verylow frequency, at rates of 10-6-10-8. Spontaneous mutations,on the other hand, are more frequent if temperature is high,like in tropical countries.In 1927 cv. Italia was introduced into Brazil, the Ferrazde Vasconcelos County, São Paulo State, by the agronomistLUCIANO POLETTI; it became the most important table grapevariety in Brazil. After its introduction some economic im-portant mutations of Italia were selected such as Rubi (1974)with purple berries (KISHINO and MASHIMA 1980); Benitaka(1988) with dark reddish berries; and Brasil (1995) with pur-ple dark fruits, a mutation of Benitaka. The nuclear DNApolymorphism through RAPD molecular markers is a simpleand inexpensive method for germplasm characterization,which has been used in several studies with grapevines(SAWAZAKI et al. 1966; THIS etal . 1997). In the present paperit was used to verify the genetic similarity between Italia andRedimeire grapevines. This study describes the mainphenotypic and biochemical characteristics of Redimeire, amutant of Italia.Material and Methods: The retromutation occurred in avineyard with Redimeire, in the Urânia County, São PauloState, Brazil, (20º16’ S and 50º24’ W, 418 m a.s.l.) when at thesame shoot we found one cluster of Redimeire, and onecluster of Italia. Leaves, shoots and fruits of Redimeire andItalia were collected for ampelographic and biochemicalanalyses; cv. Redglobe was used as an indicator of differen-tiation between cultivars. Ampelographic and ampelometricdiagnostics were conducted as proposed by GALET (1993).To characterize length, width, weight and number of seeds,10 clusters and 15 berries of each cluster of cvs Italia (I) andRedimeire (R) were sampled. For molecular diagnostic, ge-nomic DNA was extracted from a mixture of young leaves,using the Nucleon Phytopure kit of Amershan. Kits of prim-ers OPA, OPB, OPC, OPD, OPE, OPF and OPG from OPERONTechnologies were used.Results and Discussion: Observations of leaves, shootsand laterals from cvs Italia and Redimeire showed that bothcvs have the same ampelographic and ampelometric charac-teristics. The major difference between Italia and Redimeirewas found in the fruit. Italia has very large, compact, andconic-cylindrical-shaped clusters. The berries are elliptic,very large, with a medium size of 28 x 22 mm, yellow-ambercoloured, with a fleshy and crispy pulp and a slight musca-tel taste (GALET 1993). On the other hand, Redimeire hassomewhat loose clusters, with a conic-cylindrical shape. Theberries are long-oval-shaped, have medium weight, a me-dium length/width relation of 1.78, a fleshy and crispy pulp,purple coloration and a delicate muscatel taste (Fig. 1). Onaverage, the length of 100 berries of Italia was 31.3 mm, thewidth 25.5 mm; the mean weight was 11.9 g and number ofseeds 2.2. Figures for Redimeire were, respectively, 34.2 mm,19.2 mm, 7.89 g and 1.64 seeds. The coefficient of variationfor the characteristics supra, was always higher forRedimeire.Correspondence to: Dr. E. J. P. PIRES, Instituto Agronômico (IAC),Caixa postal 28, 13001-970 Campinas, SP. Brazil. E-mail:ejppires@cec.iac.brFig. 1: Berries of cvs Italia (above) and Redimeire (below).Estimation of the genetic similarity based on the numberof common DNA fragments, using the primers OPA, OPB,OPC, OPD, OPE, OPF and OPG indicated no difference be-tween Italia and Redimeire. On the other hand, differenceswere observed in relation to Redglobe with the primers OPA7,OPA8, APA11, OPA14, OPA15, OP16, OP17, OP19, OPB2,OPB13, OPC1, OPC2, OPC3, OPC4, OPC5, OPC6, OPC7, OPC8,OPD10, OPD11, OPD12, OPD13, OPD14, OPD15, OPD18,OPE1, OPE2, OPE3, OPE4, OPE5, OPE6, OPE8, OPE10, OPE16,OPE18, OPE19, OPG1, OPG5, OPG6. Fig. 2 shows thatRedglobe had different bands as compared to Italia andRedimeire with the primers OPA7, OPA8, OPA15, OPB2, OP14and OPD15.Conclusions: From ampelographic and ampelometricstudies of leaves, shoots and laterals concluded thatRedimeire and Italia are phenotypically identical cultivars.56 E. J. P. PIRES et al.From RAPD analysis it was concluded that Redimeire andItalia have the same genetic composition. Due to relevantmorphological grape characters the two cultivars are differ-ent. There were significant statistical differences for mor-phological berry characteristics, the distribution of the ber-ries characteristic being normal except for Redimeire berrywidth for which kurtosis had a very high value (30.16) farfrom normal distribution.FREGONI, M.; 2000: ‘Malvasia Rosa’ a new aromatic grapevine variety.Acta Hortic. 528, 685-687.GALET, P.; 1993: Précis de Viticulture. Imprimierie Déhan, Montpellier.6ª ed.KARNIEL, S.; 1996: Variety ‘King Husainy’ syn. ‘Jade Seedless’. Appli-cation no: 91/096. Plant Var. J. 9 (1), 17.KISHINO, A. Y.; MASHIMA, M.; 1980: In: Fundação Instituto Agronômicodo Paraná. Manual agropecuário para o Paraná, 139-177. Lon-don.Fig. 2: RAPD profile from cvs Italia (1,2), Redimeire (3,4) and Redglobe (5,6) using primers OPA7, OPA8, OPA15, OPB2, OPC14 andOPD15.MANN, D.; MANN, D. E.; 1988: Variety ‘Cygne blanc’. Application no:97/045. Plant Var. J. 11 (2), 51-52.PIRES, E. J. P.; POMMER, C. V.; PASSOS DA, I. R.; TERRA, M. M.; 1988:Seedless somatic mutant in the Niagara red grape cultivar.Bragantia 47 (2), 171-176 (portug.).SAWAZAKI, H. E.; POMMER, C. V.; PASSOS DA, I. R.; TERRA, M. M.; PIRES,E. J. P.; 1996: Identification of parents and hybrids among Vitisvinifera and Vitis rotundifolia using isozyme polymorphism andRAPD marker. Bragantia Rev. Ciencias Agronomicos 55 (2),221-230 (portug.).SOUSA, J. S. I.; 1959: Somatic mutations of the Niagara grape in Brazil.Bragantia 18 (27), 377-415 (portug.).TERRA, M. M.; PIRES, E. J. P.; RIBEIRO, I. J. A.; PASSOS, I. R. S.; 1984:Patrícia Branca: Somatic mutation in cv. Patrícia (IAC 871-41).Bragantia 43 (1), 267-270 (portug.).THIS, P.; CUISSET, C.; BOURSIQUOT, J. M.; 1997: Development of stableRAPD markers for the identification of grapevine rootstocksand the analysis of genetic relationships. Am. J. Enol. Vitic. 48,492-501.

Redimeire: A natural mutation of cv. Italia in Brazil

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Redimeire: A natural mutation of cv. Italia in Brazil

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