Methodologies based on the analysis of DNA polymorphism were recently described to identify and study grapevine rootstocks and cultivars. We report here rapid RFLP analysis of 17 Vitis, 3 Ampelopsis and 2 Parthenocissus DNAs amplified by PCR with primers based on sequences from previously defined polymorphic DNA fragments of V. vinifera cv. Chardonnay. These primers can be used as a tool for phylogenetic studies in the genus Vitis and more generally within the Vitaceae family. The DNAs of 22 rootstocks could be identified by PCR using 4 pairs of Chardonnay-derived primers combined with RFLP analysis. However, it was impossible to discriminate between 9 clones of the rootstock 3309 C. This technique is rapid and well reproducible
