This study was designed to evaluate the antibacterial & antibiofilm activity of Punica granatum peel aqueous extract against oral pathogens from patients suffering from gingivitis and dental carrier. These isolates were 58.8% gram positive (Lactobacillus spp. ,Streptococcus spp. and Staphylococcus aurus) and 41.2% gram negative (Klebseilla pneumonia and pseudomonas aeruginosa). The susceptibility of these different bacterial species toward the aqueous extract of this plant was applied by using well diffusion agar method. The results were compared with each other and with selected antibiotic ciprofloxacin as positive control. Results showed that aqueous extract of Punica granatum had antibacterial activity against all isolates, on which the diameter of inhibition zone at concentration 200 mg/ml of this extract was 24mm for Lactobacillus spp and 23 mm for Staph aureus and P.aeruginosa, while the Streptococcus spp. was only 18 mm