Meiotic behavior, pollen fertility and germination of the Brazilian table grape cv, Rubi (Vitis vinifera) were investigated; this cultivar produced different numbers of seedless berries when cultivated at two different sites: At site A vines produced a high proportion of seedless berries while at site B vines produced berries with a normal number of seeds. Cytological analysis of inflorescences collected from the two sites showed some meiotic abnormalities, the most common being related to chromosomal segregation and telophase micronuclei formation leading to microcyte formation in the tetrads, Cytoplasmic channels, bridges and tripolar spindles were also observed in some microsporocytes, Pollen fertility was high at both sites, approximately 96 % at site A and 98 % at site B, The rate of pollen germination was lower at site A than at site B, suggesting that the absence of seed formation is related to pollen germination rate.