ABSTACTThe result of the multiplex-PCR revealed positive results in 13(15.1%) of the 86 seminal fluid samples from infertile patients which represented M.hominis 5(5.8%), U.urealyticum in 5(5.8%) and U.parvum3(3.5%).Conclusion: in light of the results obtained this study we concludes that the efficiency of multiplex-PCR large and rapid in diagnosis of Ureaplasmaspp and Mycoplasma hominis in seminal fluid.Recommendation : the study of detection of other microorganism (viruses, fungi, Chlamydia and Neisseria gonorrhea) in men infertility.Key words: Multiplex-PCR,Mycoplasma, Ureaplasma,Men, Infertility
