Effect of rapid freezing and thawing on Malondialdehyde concentration , abnormal sperm chromatin percent and sperm parameters of Asthenozoospermic patients
Abstract :Objective : The study aimed to study the effect of rapid freezing and thawing on Malondialdehyde concentration andabnormal sperm chromatin percent and sperm parameters of Asthenozoospermic patientsMethodology : This study was performed in the laboratory of Intra cytoplasmic Injection and freezing of sperm in thefertility center of Al-Sader Medical City / Al-najaf Alashraf City during the period from May, 2012 to April 2013 ,The study included 30 of Asthenozoospermic patients, the statistical analysis of this study performed by usingStudent's t – test and paired t – test at the level of probability 0.05Results : the results showed that is sperm rapid freezing process of patients who were Included in the study by usedsperm freezing media ( Sperm Freeze) and doing the thawing processes after one month From freezing leads to asignificant decreasing (p<0.05) In the sperm concentration, sperm progressive motility percent, sperm viabilitypercent and normal sperm morphology percent. and a significant increasing (p<0.05) In The Malondialdehydeconcentration and abnormal Sperm chromatin percent and no significant difference(p<0.05) In round Cellconcentration in comparison with their ranges after activationConclusion : It can be concluded From this study that sperm rapid freezing and thawing processes had a negativeeffects on the semen and sperm parameters and Malondialdehyde concentration and abnormal sperm chromatinpercent In the all samples of study included patients .Recommendation: Adding Anti oxidants to the sperm freezing media to decrease the negative effects of freezingand increase the sperm parameters .Keyword : effect , rapid freezing, thawing, Malondialdehyde, chromatin, sperm parameters , Asthenozoospermi
