Aupinel, Pierrick

Decourtye, Axel

Fortini, Dominique

Fourrier, Julie

Grateau, Stéphane

Henry, Mickaël

Morhan, Clémence

Petit, Julie

Vidau, Cyril

English

JKI Open Journal Systems (Julius Kühn-Institut)

Hazards of pesticides to bees - 12th International Symposium of the ICP-PR Bee Protection Group, Ghent (Belgium), September 15-17, 2014   130  Julius-Kühn-Archiv, 450, 2015 2.10 A methodology to assess the effects of plant protection product on the homing flight of honeybee foragers Julie Fourrier1, Julie Petit, Dominique Fortini, Pierrick Aupinel, Clémence Morhan, Cyril Vidau, Stéphane Grateau, Mickaël Henry, Axel Decourtye 1ACTA, ICB-VetAgroSup, 1 avenue Bourgelat, 69280 Marcy l’Etoile, France Abstract With the current revision of plant protection product risk assessment on the honeybee by the European agency (EFSA), new methodologies are asked in the evaluation scheme (EFSA, 2013).  Homing experiments are relevant for field assessment because an increased probability of homing failure reveals a mortality phenomenon (Henry et al. 2012). Successful homing flight is contingent to the proper integration of multiple physiological and cognitive functions (navigation, memory, energetic metabolism and muscular flight activity). We developed and finalised a methodology based on RFID (Radio-Frequency IDentification) technology already valued in ecotoxicology (working group of CEB). The standardisation of the method is conducted by the French institute of beekeeping and pollination (ITSAP).We now have the will to validate the method by creating an international ring test group with interested laboratories for a registration in the OECD guidelines. A homing trial is defined as a group of forager bees released at one given site after receiving an acute oral insecticide or control treatment. To ascertain they had a prior knowledge of the pathway back to the colony, foragers with bright blue pollen loads from a known Phacelia field were captured at the entrance of the hive. Phacelia was planted in a one-ha field specifically for the need of the experiment, and the colony subsequently placed 1 km away. Bees were each labelled with microchips and orally exposed to a sublethal dose of insecticide or to a control in laboratory. The dose was administered to bees in 20 µl of a 30% sucrose solution. Then, tagged foragers were released from inside the Phacelia field. Homing failure is defined as an absence of a RFID record during the 24-h post-release.  References EFSA. Guidance on the risk assessment of plant protection products on bees (Apis mellifera, Bombus spp. and solitary bees). EFSA J. 11, 3295 (2013). Henry, M. et al. A common pesticide decreases foraging success and survival in honey bees. Science 336, 348–350 (2012). CEB method n° 230. Evaluation of the effects of plant protection product on honeybees. Evaluation of the effects of plant protection products on the homing flight performances of honeybees.  Acknowledgment This work is funded by the European Community program coordinated by the French Ministry of Agriculture (FranceAgriMer).   

A methodology to assess the effects of plant protection product on the homing flight of honeybee foragers

https://ojs.openagrar.de/index.php/JKA/article/download/5329/5074

A methodology to assess the effects of plant protection product on the homing flight of honeybee foragers

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