Herbal preparations in Siddha system of medicine always have a unique range of beneficial effect because of its wonderful therapeutic value without causing adverse effects. Here the drug Thathu Viruthi Chooranam was pharmacologically evaluated and scientifically validated and standardized.
The ingredients of the drug was identified and authenticated by Gunapadam experts. The drug was prepared as per classical Siddha literary procedure and subjected to various studies to reveal its potency and efficacy of the drug.
The organoleptic character and physicochemical studies were made into standardization of the drug TVC. From the above studies the TVC is standardized as per AYUSH guidelines.
The biochemical and instrumental analysis was made to know the presence of active ingredients in the drug which is responsible for its activity.
Here, the biochemical analysis showed the presence of Calcium, Magnesium, Zinc, Sulphate, Chloride by its synergistic effects, the drug as activity against the disease.
In instrumental analysis, FTIR showed the OH group has higher potential towards inhibitory activity against microorganisms. Sometimes the presence of Phenols in medicinal plants possess highly Anti-Oxidant property which enhances the drug effect against the disease.
SEM picture explained the particle size of the drug. In ICP-OES described about the absence of heavy metals and its permissible limits which showed the safety of the drug.
TLC plate showed different colour phytoconstituents of chloroform extract of TVC. The bands revealed presence of seven violets, four reds, two greens, one blue, one white and one yellow showing the presence of alkaloids, glycosides, phenols, triterpenes, flavonoids and quinines.
The drug Thathu Viruthi Chooranam was proved that it is free from toxicity through the acute and 28 days repeated oral toxicity study as per the OECD guidelines. In acute toxicity study there was no mortality of rats observed. In 28 days repeated oral toxicity study, the obtained results of haematological, biochemical, urinary parameters were normal. The histopathological findings did not show any abnormalities.
And this herbal formulation possess more potent Aphrodisiac activity in ethanol treated male rats. This trial drug Thathu Viruthi Chooranam exhibits high range of Spermatogenic activity in ethanol induced male rats.
Most of the research findings already reported that the role of antioxidant is essential to treat male infertility. Here the trial drug possess strong antioxidant activity.
These results conclude that the drug Thathu Viruthi Chooranam is a best drug of choice for the treatment of male infertility.
By analyzing all those findings, it is proved that the drug Thathu Viruthi Chooranam has high range of therapeutic value and the safety of the drug for clinical use is ensured. So, it is confirmed that the herbal formulation Thathu Viruthi Chooranam may never cause any adverse effects in clinical use. The preparation of the drug is cost effective too.
So, the clinical trials have to be followed on the drug Thathu Viruthi Chooranam. Thus can treat the infertile males effectively and help them to achieve their dream of producing their own offspring.
CONCLUSION:
The drug “Thathu Viruthi Chooranam” was prepared as per the classical Siddha literature Sarabenthirar Vaithiya Rathnavali.
It fulfills all the standardization parameters of Chooranam as mentioned in AYUSH guidelines.
The results of biochemical analysis showed the presence of acid and basic radicals in the sample.
The presence of alkaloids, flavonoids, glycosides, tannins, triterpenoids were identified in the choloroform and methanolic extract of Thathu Viruthi Chooranam through Phytochemical analysis.
TLC plate showed different colour phytoconstituents of chloroform extract of TVC.
FT-IR Study results exhibits the presence of some organic functional groups such as alcohols, phenols, alkenes, amines, aromatics, aliphatic amines, alkyl halides.
SEM analysis showed the objects of sizes ranging from 3μm to5 μm.
XRD pattern of the trial drug Thathu Viruthi Chooranam shows good crystalinity.
Based on OECD 423 the trial drug Thathu Viruthi Chooranam is considered as non toxic up to the dose of 2000mg/kg.
The drug Thathu Viruthi Chooranam possess the potent Aphrodisiac activity in ethanol treated male rats.
The drug Thathu Viruthi Chooranam could be confirmed as no-observedadverse-effect level (NOAEL) drug as it acts harmlessly under the current normal usage and this phenomenon is considered to be of no toxicological concern.
High antioxidant therapeutic nature was evaluated in the drug Thathu Viruthi Chooranam through DPPH Scavenging assay. Because of this anti-oxidant property, it can be used as a drug to treat the male infertility.
Hence it is proved that the drug Thathu Viruthi Chooranam was pharamacollogicaly evaluated for the property of Spermatogenic, Aphrodisiac and Antioxidant activity
