RP-HPLC Method Development and Validation for Estimation of Related Substances of Acetaminophen in Acetaminophen, Phenylephrine Hcl and Dextromethorphanhbr Soft Gelatin Capsules.

Abstract

Analytical chemistry is measurement science consisting of a set of powerful ideas and methods that are useful in all fields of science and method. it is used to study the chemical composition, structure and behavior of compounds. The purpose of chemical analysis is together and interpret chemical information that will be the value of society in a wide range of contexts. involves the application of a range of techniques and methodologies to obtain and assess qualitative, quantitative and structural information on the nature of components. the aim of present work, the RP-HPLC method was developed to estimate the related substances of acetaminophen in acetaminophen, phenylephrine Hcl and dextromethorphan HBr in soft gelatin capsules. From the result of validation parameters obtained, it was observed that the developed method was proven to be specific, precise, linear, accurate, rugged and robust and is suitable for its intended purpose. Hence it was concluded that this method could be used for the routine estimation of related substances of acetaminophen in acetaminophen, phenylephrine Hcl, dextromethorphan HBr in soft gelatin capsules