The purpose of this study was to examine the relationship between the motion of the trunk and lower extremities and ball velocity during the overhand throw in canoe polo. Fifteen male national canoe polo team players participated in this study. The overhand throwing motion was captured using a three dimensional motion analysis system. Kinematic and temporal parameters in the trunk and lower extremities were measured and analyzed. Results indicated that five variables were associated with variations in ball velocity. Specifically, as ball velocity increased, canoe polo players showed an increased maximal angular velocity in trunk-tilt sideways, upper torso rotation, and right knee flexion. In addition, the right knee flexion range of motion and time to maximum right knee flexion angular velocity increased as ball velocity increased