University of Zagreb Faculty of Food Technology and Biotechnology
Abstract
Geotrichum candidum Geo17 was cultivated on peptones as carbon and nitrogen source, and in the presence of lactate as the second carbon source. From the analysis of the initial and final culture medium after total hydrolysis, the yield of consumption was determined for each amino acid. Amino acids have been considered a convenient carbon source for biosynthesis, while the rest of the amino acids were assumed to be used only as a nitrogen source, with the corresponding carbon released as CO2 resulting from energy supply. Carbon mass balances confirmed this assumption. A clear differentiation between the amino acids assimilated as carbon sources and those assimilated as energy sources was therefore highlighted