アフリカツメガエル卵母細胞へのRNA, DNA等の細胞成分注入用の簡易な微量注入装置を考案, 試作した。この装置を用い特に植物性mRNAをアフリカツメガエル卵母細胞へ注入しタンパク質生合成を行わせた。その結果, tobacco mosaic virus RNA, brome mosaic virus RNA, イネglutelin前駆体mRNA, エンドウriburose 1,5 bis-phosphate carboxylase mRNAが忠実に翻訳され, 対応するpolypeptideが形成されることをsodium dodecyl sulfate polyacrylamide gel電気泳動(SDS-PAGE)による分子量の測定と免疫学的方法を組合わせることにより確認した。A simplified and convenient microinjection system to inject cell components such as DNA and RNA into Xenopus oocytes was devised and constructed. By use of this system, RNAs of plants were injected into Xenopus oocytes, then their protein syntheses were examined. Tobacco mosaic virus RNA, brome mosaic virus RNA, rice glutelin precusor mRNA, and pea Riburose 1,5bisphosphate carboxylase mRNA were faithfully translated. The nature of the synthesized polypeptide was confirmed by fluorography using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoelctrophoresis
