It has been increasingly recognized that cancer patient\u27s genetic information significantly influences therapeutic outcomes. In this study, a novel experimental system was developed for estimating risk of radiation-induced normal tissue injury on individual cancer patients based on analysis of marker SNP genotypes, which had been screened by a large association study on genes involved in DNA damage repair, cell cycle control, redox regulation, inflammation and so on. These marker SNPs were analyzed simultaneously using patient\u27s blood-derived genomic DNA by a novel DNA chip method that provided optical detection of typing results. The resulting spot images on the DNA chips were captured by a digital camera and processed by computerized tool to call genotype of SNPs. The genotype information was then referred a database made by the foregoing large association study to statistically calculate risk of radiation-induced normal tissue injury. This system does not require specific instruments and can be easily operated in a short time. It is suitable to be used at laboratory of clinical hospitals.Cancer Models & Mechanism
