Chiono, Valeria

Ciardelli, Gianluca

Idrees, Ayesha

Salber, Jochen

Viebahn, Richard

English

Ayesha Idrees

Valeria Chiono

Richard Viebahn

Gianluca Ciardelli

Jochen Salber

PORTO@iris (Publications Open Repository TOrino - Politecnico di Torino)

04 August 2020POLITECNICO DI TORINORepository ISTITUZIONALEComparative cytocompatibility evaluation: two-dimensional (2D) vs. three-dimensional (3D) cell culture tools / Idrees,Ayesha; Chiono, Valeria; Viebahn, Richard; Ciardelli, Gianluca; Salber, Jochen. - (2018). ((Intervento presentato alconvegno GNB 2018 - Sixth National Congress on Bioengineering tenutosi a Politecnico di Milano, Italy nel June 2018.OriginalComparative cytocompatibility evaluation: two-dimensional (2D) vs. three-dimensional (3D) cell culturetoolsPublisher:PublishedDOI:Terms of use:openAccessPublisher copyright(Article begins on next page)This article is made available under terms and conditions as specified in the  corresponding bibliographic description inthe repositoryAvailability:This version is available at: 11583/2710540 since: 2019-01-11T18:48:10ZNot applicableComparative Cytocompatibility Evaluation: Two-Dimensional (2D) Vs. Three-Dimensional (3D) Cell Culture ToolsAyesha Idrees1,2,3, Valeria Chiono1, Richard Viebahn3, Gianluca Ciardelli1, Jochen Salber2,31Department of Mechanical and Aerospace Engineering (DIMEAS), Politecnico di Torino, Italy2Medical Biomaterials, Center for Clinical Research of the Ruhr-University Bochum, Germany3UMC Knappschaftskrankenhaus GmbH - Hospital of the Ruhr-University Bochum, GermanyAbstract— Wound management still represents a significant problem. Furthermore, infection of wounds leads to non-healing conditions. With an increasing need of novel strategies for wound healing as well as treatment of infected wounds, there is an extreme urgency to develop reliable in vitro tools for biological evaluation. 3D cell culture has a potential to provide a more physiologically relevant model compared to standard 2D cell culture. For this purpose, the cytocompatibility and antimicrobial activity of different silver-based species occurring in commercially available wound dressings were investigated in this study, comparing the responses of 2D monolayer and 3D cell culture-based testing systems.Keywords—3D cell culture, 2D cell culture, wound dressings, in vitro tools.Abstract— Wound management still represents a significant problem. Furthermore, infection of wounds leads to non-healing conditions.With an i creasing n ed of novel strategies for wound heali g as well a tre tm nt of infected wounds, there is n extreme urge cy todevelop reliable in vitro tools for biological evaluation. 3D cell culture has a potential to provide a more physiologically relevant modelcompared to standard 2D cell culture. For this purpose, the cytocompatibility and antimicrobial activity of different silver-based speciesoccurring in commercially available wound dressings were investigated in this study, comparing the responses of 2D monolayer and 3Dcell culture-based testing systems.020406080100Percent Viability ofIn-vitro Human Skin (%)Decreasing concentration of Ag+ 020406080100Ag+ MBC Ag+ wounddressingPercent Reduction of S. aureus in Liquid Broth CFU assay (%)77.47.88.28.699.49.8Ag+ MBC Ag+ wounddressingUntreatedLog CFU per S. aureus Colonized In-vitroHuman Skin (Log) 020406080100Ag+wounddressingWounddressingcontrol(WithoutAg+)UntreatedcontrolPercent Viability of In-vitroHuman Skin  (%)020406080100120Percent Viability  of Human Keratinocytes in 2D (%)Abstract— Wound management still represents a significant problem. Furthermore, infection of wounds leads to non-healing conditions. With an increasing need of novel strategies for wound healing as well as treatment of infected wounds, there is an extreme urgency to develop reliable in vitro tools for biological evaluation. 3D cell culture has a potential to provide a more physiologically relevant model compared to standard 2D cell culture. For this purpose, the cytocompatibility and antimicrobial activity of different silver-based species occurring in commercially available wound dressings were investigated in this study, comparing the responses of 2D monolayer and 3D cell culture-based testing systems.Keywords—3D cell culture, 2D cell culture, wound dressings, in vitro tools.Introduction: Wounds with delayed healing are emerging as serious problems in the clinics causing aconsiderable morbidity and a high cost of health care. With emerging novel materials for wound management,there is an extreme need to develop reliable in vitro tools for biological evaluation [1], [2]. Monolayers ofadherent c lls have been used for in vitro t sti g for decades. However, ey are ass ciated with differe tlimit tions, mainly becaus of their inability to mimic in vivo conditions. Three-di ension l (3D) cell culturesystems are more physiologically relevant and extremely needed for cytocompatibility testing of biomaterials.Non-healing wounds are mainly associated with infections of the wounds causing a chronic and non-healingcondition. The 3D cell culture system developed in this study was further used to generate a 3D infected woundmodel, to better mimic in vivo infected wound conditions. Considering the emergence of antibiotic resistance,this system will serve as an advanced tool to screen novel antimicrobial biomaterials for wound healing.Pat. with an infected wound at thelateral right side of the lower leg.Central part of the highly inflamedpressure ulcer is necrotic. Bacteriafound in the wound was MRSA.In-vitro Human Skin Model S. aureus Colonized Human Skin ModelAcknowledgements: HyMedPoly received funding from the European Union’s Horizon 2020 research and innovation programmeunder the Marie Sklodowska‐Curie grant agreement No 643050. The authors thank all the HyMedPoly Partners in this HyMedPoly project.020406080100Percent Viability  of Human Keratinocytes  in 2D (%)Cytocompatibility Analysis: Skin Cells (2D Cell Culture) Vs. In-vitro Human Skin Model (3D Cell Culture System)Antibacterial AnalysisAbstract— Wound management still represents a significant problem. Furthermore, infection of wounds leads to non-healing conditions. With an increasing need of novel strategies for wound healing as well as treatment of infected wounds, there is an extreme urgency to develop reliable in vitro tools for biological evaluation. 3D cell culture has a potential to provide a more physiologically relevant model compared to standard 2D cell culture. For this purpose, the cytocompatibility and antimicrobial activity of different silver-based species occurring in commercially available wound dressings were investigated in this study, comparing the responses of 2D monolayer and 3D cell culture-based testing systems.Keywords—3D cell culture, 2D cell culture, wound dressings, in vitro tools.Conclusion: Before testing new materials in clinical trials, predictive and reliable preclinical data are required. Taking this into consideration, responses of a 3D system in comparison to 2D monolayer cells were performed to provide more reliable preclinical outcomesThis system was generated by first developing the dermal compartment by embedding human dermal fibroblasts inrat tail tendon collagen type 1 as extracellular matrix (ECM). Once the ECM was remodelled by human fibroblasts,human epidermal keratinocytes were seeded on it and allowed to differentiate by lifting the culture to an air liquidinterface (ALI). The fully differentiated 3D human skin model was characterized by histology staining,immunohistochemistry and physicochemistry.The 3D human skin model was further developed as an infected skin model by colonizing S.aureus at wound site.

Comparative cytocompatibility evaluation: two-dimensional (2D) vs. three-dimensional (3D) cell culture tools

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Comparative cytocompatibility evaluation: two-dimensional (2D) vs. three-dimensional (3D) cell culture tools

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