ABSTRACT. This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation – Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_2874 and Mrub_1349. We predict that Mrub_2874 encodes the enzyme ribulose-5-phosphate 3-epimerase (DNA coordinates 2912530..2913204 on the reverse strand), which is the first step of the pentose phosphate pathway (KEGG map number 00030). It catalyzes the conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate. The E. coli K12 MG1655 ortholog is predicted to be b3386, which has the gene identifier rpe. We predict that Mrub_1349 encodes the enzyme ribose-5-phosphate isomerase (DNA coordinates 1381292..1381978 on the reverse strand), which is also the first step of the pentose phosphate pathway (KEGG map number 00030). It catalyzes the conversion of D-ribulose 5-phosphate to D-ribose 5-phosphate. The E. coli K12 MG1655 ortholog is predicted to be b2914 and b4090, which have the gene identifiers rpiA and rpiB respectively. The comparison of Mrub_2875 to b3386 and the comparison of Mrub_1349 to b2914 were conclusive, but the data was inconclusive when comparing Mrub_1349 to b4090