Results of antimicrobial action of electrochemically activated water (anolyte/catholyte) are provided. The two types of water are produced in the anode and cathode chamber of an electrolitic cell, respectively. Under laboratory conditions cell culture and organ suspensions of Classical swine fever virus were treated with anolyte. By inoculating them with cell cultures quality viral presence (presence of viral antigen) was reported using the immunoperoxidase technique. It was found that: anolyte did not affect the growth of the cell culture PK-15; viral growth in the infection of a cell monolayer with a cell culture virus was affected in the greatest degree by anolyte in 1:1 dilution and less by the other dilutions; viral growth in the infection of a cell suspension with cell culture virus was affected by anolyte in dilution 1:1 in the greatest degree, and less by the other dilutions; viral growth in the infection with a virus in organ suspension of a cell monolayer was affected by the anolyte in all dilutions applied. Unexpectedly, stronger biocidal effect of the catholyte was obsrved when E. coli DH5a strain was treated by anolyte and catholyte, respectively. In order to provide additional information about the antiviral activity of the electrochemically activated water, and about the distribution of water molecules according to the energies of the hydrogen bonds, the non-equilibrium energy spectrum (NES) and diferential non-equilibrium energy spectrum (DNES) of anolyte and catholyte is evaluated. Keywords: anolyte, cell culture, CSF virus, disinfection, hydrogen bond, water, structure, clusters, NES, DNE
