One Health in terms of collaboration, particularly between human and animal health sectors to prevent and control zoonoses has been low while the sectors have a lot of things in common. Such common things include aspects of disease causative agents (viruses, bacteria, parasites, etc.) and those of disease occurrence mediator conditions (social, cultural, economic or climatic). Therefore, the research from which this paper is based was done with the objectives to: (a) assess the extent to which human and animal health policies facilitate one health in terms of collaboration; (b) rank opportunities for and challenges to collaboration among medical, and veterinary officers according to the views and experiences of the respondents in the Ministry of Health and Ministry of Agriculture; and (c) determine the attitude of the respondents towards One Health approaches in terms of collaboration in dealing with zoonoses. A cross-sectional research design was used in this study whereby data were collected at a single point in time without repetition. Purposive sampling method was used to make sure that the respondents were only officials who usually participated in policy formulation in the two Ministries. It was found that almost three quarters (73.1%) of the respondents from both ministries agreed that there was no policy which directly facilitated One Health in terms of collaboration. It was also found that 83.6% of the respondents pointed out that human and animal health policy making process was a top-down process. Furthermore, it was found that the main opportunities that could enhance collaboration were sufficient money in budgeting; advocacy for control of neglected zoonotic diseases in human and animal health; and one health policy formulation (71.3%, 68.2% and 65.5% respectively). The overall attitude towards collaboration among respondents was favourable; they scored an average of 62.2 out of 100.0 points on a Likert scale. It is concluded that if opportunities enhancing collaboration were strengthened and challenges to collaboration were overcome, human health and animal health experts could collaborate more in reduction of disease burden in both humans and livestock. Keywords: One health, policy, attitude, opportunities, challenge